催化荧光法测定注射用头孢哌酮钠含量

Catalytic Fluorimetric Determination of Cefoperazone Sodium in Injection

摘要: 在氢氧化钠介质中, 头孢哌酮钠能催化过硫酸钾氧化光泽精褪色,使体系荧光猝灭,据此建立了测定头孢哌酮钠含量的催化荧光分析方法.头孢哌酮钠的质量浓度与荧光强度的变化在7.56～55.44 mg /L范围内呈良好的线性关系，相对标准偏差(RSD)为2.41%，方法的检出限(S/N=3)为6.95 mg/L.该法简便、快速，可靠，并应用于药物粉针制剂中头孢哌酮钠含量的测定，结果令人满意.

Abstract: A new catalytic fluorimetric method for the determination of trace cefoperazone sodium has been developed. The method is based on the catalytic effect of cefoperazone sodium on the oxidation of K₂S₂O₈ with lucigenin in NaOH medium. The detection limit(S/N=3) is 6.95 mg /L and the linear range of determination is 7.56～55.44 mg /L . The proposed method is simple 、speediness and credibility . The method has been applied to the determination of cefoperazone sodium in injection.