高效液相色谱法定量测定采油微生物发酵菌液中的有机酸

HPLC Determination of the Organic Acid Content inFermentation Liquor of In-situ-biological Process for Oil Production

摘要: 采用反相高效液相色谱法（RP-HPLC）在Kromasil C18分析柱上，以0.015 mol/L磷酸二氢钠缓冲液（pH=2.3）和乙腈（体积比为96∶4）的二元流动相分离测定了采油微生物发酵液中的有机酸，流动相的流速为1.0 mL/min，采用紫外检测器在210 nm处对有机酸进行测定.结果表明有机酸浓度在0.05～1.5 g/L时其峰面积与相应的质量浓度有良好的线性关系，其r大小等于0.9990，测定结果的RSD都小于4.0％(n=8)，平均回收率均为100±4％以内.该方法具有测定结果准确可靠，操作简便快速，重现性好等特点.

Abstract: Using duality mobile phase of sodium dihydrogenphosphate buffer solution (pH=2.3) and acetonitrile （96∶4，V/V）, we could determine the content of organic acid in fermentation liquor by reversed\|phase high performance liquid chromatographic method. Flow rate of mobile phase was 1.0 mL/min with UV-detection at 210 nm.The results showed that while the contents of organic acids were in the range of 0.05～1.5 g/L,the area and the content were in a well linear correlation(r≥0.9990,RSD<4.0%(n=8)), the average recovery was 100％±4％. This method also had the simple, rapid, well accuracy.