杨洁, 严复. 高效液相色谱法测定镇咳药中左旋羟基苯哌嗪含量[J]. 分析测试技术与仪器, 2007, (3): 215-218.
引用本文: 杨洁, 严复. 高效液相色谱法测定镇咳药中左旋羟基苯哌嗪含量[J]. 分析测试技术与仪器, 2007, (3): 215-218.
YANG Jie, YAN Fu. Determination of Levodropopizine in Anti-tussive Drug by HPLC[J]. Analysis and Testing Technology and Instruments, 2007, (3): 215-218.
Citation: YANG Jie, YAN Fu. Determination of Levodropopizine in Anti-tussive Drug by HPLC[J]. Analysis and Testing Technology and Instruments, 2007, (3): 215-218.

高效液相色谱法测定镇咳药中左旋羟基苯哌嗪含量

Determination of Levodropopizine in Anti-tussive Drug by HPLC

  • 摘要: 建立了高效液相色谱法(HPLC)测定镇咳药中左旋羟基苯哌嗪含量的方法,运用二极管阵列检测器(PAD),C18反相柱,以甲醇∶水(冰醋酸HAc调节pH值为6.5)∶三乙胺(TEA)(30∶70∶0.5,V/V/V),为流动相测定了3批镇咳药(利咳净糖浆)中左旋羟基苯哌嗪的含量分别为5 772.6、5 809.2、5 833.2 μg/mL,变异系数小于2%,回收率为99.1%~100.2%.该方法快速,精密度及准确度在允许范围内,可作为镇咳药中左旋羟基苯哌嗪的测定方法.

     

    Abstract: A HPLC method for determination of Levodropopizine in anti-tussive drug was reported, The sample was analyzed on a C18 reversed-phase column (5 μm, 200×4.6 mm) with a isogradient mobile of methanol: water(HAC adjest pH=6.5): TEA(30∶70∶0.5,V/V/V). Levodropopizine in 3 baths Levodropropizine Syrup was tested .The contents of LDP were 5 772.6、5 809.2、5 833.2 μg/mL respectively, variation coefficient was less than 2%, and the recovery was 92.51%~99.30%.This method is simple, sensitivity, reproducibility and can be used for the determination of LDP in anti-tussive drug.

     

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