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韩瑞丽,马健,张佳程,生庆海. HPLC-ELSD与GC-MS法测定牛乳甘油三酯sn-2位脂肪酸组成[J]. 分析测试技术与仪器, 2009, 15(1).
引用本文: 韩瑞丽,马健,张佳程,生庆海. HPLC-ELSD与GC-MS法测定牛乳甘油三酯sn-2位脂肪酸组成[J]. 分析测试技术与仪器, 2009, 15(1).
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HAN Rui-li1,MA Jian2,ZHANG Jia-cheng1,SHENG Qing-hai3. Determination of sn-2 Fatty Acid in Triglyceride of Milk by HPLC-ELSD and GC-MS[J]. Analysis and Testing Technology and Instruments, 2009, 15(1).
Citation: HAN Rui-li1,MA Jian2,ZHANG Jia-cheng1,SHENG Qing-hai3. Determination of sn-2 Fatty Acid in Triglyceride of Milk by HPLC-ELSD and GC-MS[J]. Analysis and Testing Technology and Instruments, 2009, 15(1).
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HPLC-ELSD与GC-MS法测定牛乳甘油三酯sn-2位脂肪酸组成

Determination of sn-2 Fatty Acid in Triglyceride of Milk by HPLC-ELSD and GC-MS

    摘要
  • 摘要: 研究建立了快速\准确测定牛乳脂甘油三酯sn-2位脂肪酸组成的方法,利用胰酶专一水解甘油三酯sn-1和sn-3位置上的脂肪酸得到sn-2单甘油酯和游离脂肪酸,再通过蒸发光散射高效液相色谱分离出sn-2位单甘油酯,然后对其进行衍生,用气相色谱质谱联用仪对sn-2脂肪酸组成进行分析,结果显示用蒸发光散射高效液相色谱法分离sn-2位单甘油酯时方法的回收率达到83.3%~85.1%,该法省去了传统测定中费时费力的薄层色谱分离步骤.用气相色谱质谱联用法对产物进行分析,精密度高,结果可靠,分析结果表明,牛乳脂肪sn-2位脂肪酸由2.57%月桂酸、7.68%豆蔻酸、34.74%棕榈酸、11.56%亚油酸、22.53%油酸和15.21%硬酯酸组成.

     

    Abstract: A quick and sensitive method has been developed for the determination of the composition of sn-2 fatty acid in milk fat.Free fatty acids and 2-monoacyl-sn-glycerols were obtained from milk triglyceride upon hydrolysis of the acyl groups in the 1-and 3-positions of glycerol using porcine pancreatic lipase.Then 2-monoacyl-sn-glycerols was separated by high-performance liquid chromatography-evaporative light scattering detector(HPLC-ELSD),the 2-position fatty acid composition was determined by gas chromatograp...

     

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