泛昔洛韦解离常数和结合常数的测定

Determination of Dissociation Constant and BindingConstant of Famciclovir

摘要: 采用紫外分光光度法，在225 nm 与305 nm波长处测得泛昔洛韦的解离常数pK_a均为3.8.在含有泛昔洛韦的pH值为7.0的Na₂HPO₄-KH₂PO₄缓冲溶液中加入牛血清白蛋白，以双倒数公式法测定二者的结合常数.结果表明，牛血清白蛋白与泛昔洛韦形成了1∶4的配合物，其结合常数是4.51×10⁶ L/mol.方法简便，结果可靠.

Abstract: Both the dissociation constants of famciclovir obtained were 3.8 using ultraviolet spectrophotometry with the wavelengths at 225 nm and 305 nm. Bovine serum albumin was added to a Na₂HPO₄-KH₂PO₄ buffer solution (pH 7.0) with famciclovir. The binding constant was measurd by the double-reciprocal analysis. The results showed that bovine serum albumin and famciclovir formed a 1∶4 complex, and the binding constant obtained was 4.51×10⁶ L/mol. This method is simple and reliable.