宁生荣, 裴栋, 冯祖飞, 封士兰, 邸多隆. 中药普乐安片质量标准研究[J]. 分析测试技术与仪器, 2011, 17(2): 83-87.
引用本文: 宁生荣, 裴栋, 冯祖飞, 封士兰, 邸多隆. 中药普乐安片质量标准研究[J]. 分析测试技术与仪器, 2011, 17(2): 83-87.
NING Sheng-rong, PEI-Dong, FENG Zu-fei, FENG Shi-lan, DI Duo-long. Study on Quality Standard for Pule′an Tablet[J]. Analysis and Testing Technology and Instruments, 2011, 17(2): 83-87.
Citation: NING Sheng-rong, PEI-Dong, FENG Zu-fei, FENG Shi-lan, DI Duo-long. Study on Quality Standard for Pule′an Tablet[J]. Analysis and Testing Technology and Instruments, 2011, 17(2): 83-87.

中药普乐安片质量标准研究

Study on Quality Standard for Pule′an Tablet

  • 摘要: 建立中药普乐安片的质量标准.采用TLC法对普乐安片中主要有效成分山奈酚-3-O-β-D-(2-O-β-D-葡萄糖基)吡喃葡萄糖苷(KMP)进行定性鉴别.采用HPLC法对普乐安片中主要有效成分山奈酚-3,4′-双-O-β-D-葡萄糖苷(KMG)和山奈酚-3-O-β-D-(2-O-β-D-葡萄糖基) 吡喃葡萄糖苷进行含量测定.在优选的TLC条件下,可定性鉴别普乐安片中KMP.在优选的HPLC条件下,KMG在0. 011 2~2.244 0 mg/mL范围内线性关系良好,r为0.999 7,平均回收率为97.7%,RSD为1.39%;KMP在0. 011 6~2.236 0 mg/ mL范围内线性关系良好,r为0.999 6,平均回收率为103.3%,RSD为1.51%.所建立方法可靠、准确、专属性强,可用于普乐安片的质量控制.

     

    Abstract: A quality standard for Pule′an Tablet is established. The qualitative identifications of Kaempferol-3-O-β-D-(2-O-β-D-glucosyl)glucopyranoside(KMP) in Pule′an Tablet were performed by TLC; Kaempferol-3,4′-di-O-β-D-glucoside (KMG) and Kaempferol 3-O-β-D-(2-O-β-D-glucosyl)glucopyranoside(KMP) in the formulation were quantified by HPLC. Under optimal conditions, KMG in Pule′an Tablet can be qualitatively and quantitatively identified. Calibration curves of KMG and KMP were found to be linear with in the ranges of 0. 011 2~2.244 0 and 0.011 6~2.236 0 mg/mL, respectively . The correlation coefficient of the calibration curve for the KMG and KMP were 0.999 7 and 0.999 6; the average recoveries were 97.7% and 103.3%, with RSD 1.39% and 1.51%, respectively. This reliable, accurate and specific method can be used for the quality control of Pule'an Tablet.

     

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