Abstract: Simultaneous determination of seven estrogens residue was developed using liquid chromatography tandem mass spectrometry. Under the optimal conditions, target compounds presented good linear relationship with correlation coefficients larger than 0.995 7 and limit of detections in the range of 0.500 0~50.00 ng/mL. Fish tissue was extracted by a C₁₈-solid phase extraction cartridge, washed with 4.0 mL of acetonitrile-H₂O (5:95, V/V,%), and then eluted with 2.0 mL of acetonitrile-H₂O (95:5, V/V,%). Both bisphenol A and dienestrol were detected in three different fish samples, while estradiol and estrone were detected in part of the samples. Recoveries were in the range of 85.70%~120.9%.The proposed method is rapid, accurate and suitable to analyze diverse residue of estrogens in aquatic products.