黄端华, 张兰. 毛细管区带电泳法测定3种香豆素的离解常数[J]. 分析测试技术与仪器, 2016, 22(1): 9-14. DOI: 10.16495/j.1006-3757.2016.01.002
引用本文: 黄端华, 张兰. 毛细管区带电泳法测定3种香豆素的离解常数[J]. 分析测试技术与仪器, 2016, 22(1): 9-14. DOI: 10.16495/j.1006-3757.2016.01.002
HUANG Duan-hua, ZHANG Lan. Determination of Dissociation Constants of Cumarins by Capillary Zone Electrophoresis[J]. Analysis and Testing Technology and Instruments, 2016, 22(1): 9-14. DOI: 10.16495/j.1006-3757.2016.01.002
Citation: HUANG Duan-hua, ZHANG Lan. Determination of Dissociation Constants of Cumarins by Capillary Zone Electrophoresis[J]. Analysis and Testing Technology and Instruments, 2016, 22(1): 9-14. DOI: 10.16495/j.1006-3757.2016.01.002

毛细管区带电泳法测定3种香豆素的离解常数

Determination of Dissociation Constants of Cumarins by Capillary Zone Electrophoresis

  • 摘要: 采用毛细管区带电泳法(CZE)测定瑞香苷(DN)、瑞香素(DP)和7-羟基香豆素(7-HC)的离解常数(pKa).通过测试不同酸度条件下各香豆素的迁移时间, 以香豆素在电泳中有效淌度和运行液中OH-浓度的关系, 推导了毛细管区带电泳-紫外分光光度(CZE-UV)法测定3种香豆素离解常数(pKa)的线性模型, 并建立了这3种香豆素的pKa测定方法.测得3种香豆素的pKa值分别是7.43(7-HC)、6.91(DN)和6.86(DP).同时也采用传统方法-紫外分光光度法对这些物质的pKa进行测定以验证CZE的可靠性, 两种测定结果相一致, 说明CZE-UV法用于3种香豆素的离解常数的测定, 快速、简便、结果可靠.

     

    Abstract: Capillary zone electrophoresis(CZE) is applied to the determination of dissociation constants of coumarin derivatives (daphnin, daphnetin and umbelliferone). Capillary electrophoresis is an attractive method for pKa measurements, which is based on the migration time or mobilities of ionic species over a range of pH values. The pKa values of three cumarins is 7.43(7-HC), 6.91(DN) and 6.86(DP). The method was validated by the comparison of results obtained by a traditional method, UV spectroscopy. No distinct difference was observed between the two methods. The CZE-UV method for pKa measurement shows good repeatability and reliability.

     

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