徐秀伟, 黄延俊. 荧光法测定糖果中的总糖含量[J]. 分析测试技术与仪器, 2016, 22(1): 23-27. DOI: 10.16495/j.1006-3757.2016.01.004
引用本文: 徐秀伟, 黄延俊. 荧光法测定糖果中的总糖含量[J]. 分析测试技术与仪器, 2016, 22(1): 23-27. DOI: 10.16495/j.1006-3757.2016.01.004
XU Xiu-wei, HUANG Yan-jun. Detection of Total Sugar in Candy by Fluorescence Spectrometry[J]. Analysis and Testing Technology and Instruments, 2016, 22(1): 23-27. DOI: 10.16495/j.1006-3757.2016.01.004
Citation: XU Xiu-wei, HUANG Yan-jun. Detection of Total Sugar in Candy by Fluorescence Spectrometry[J]. Analysis and Testing Technology and Instruments, 2016, 22(1): 23-27. DOI: 10.16495/j.1006-3757.2016.01.004

荧光法测定糖果中的总糖含量

Detection of Total Sugar in Candy by Fluorescence Spectrometry

  • 摘要: 合成了带有荧光基团的分子探针, 光谱显示探针对糖类物质具有较强的荧光响应.通过条件优化, 选取328 nm作为荧光激发波长, pH 10.0的缓冲溶液为最佳测定介质, 当测试溶液中葡萄糖浓度为0.05~1 mmol/L时, 显示良好的响应线性.方法对果糖中总糖含量测定的检测限为6.2 μmol/L, 回收率在76.4%~90.0%, RSD达到3.18%.

     

    Abstract: In order to overcome the complicated operation and relatively low sensitivity of conventional method, a method to determine the total sugar of candy by fluorescence spectrometry has been established. The procedure started with the synthesis of a molecular probe bearing pyrene fluorephore, which shows strong fluorescent response towards carbohydrates. After optimization of the experiment, the determinaton of glucose concentraion was performed in a pH of 10.0 aqueous buffer, with the assay solution being excited at 328 nm. The calibration curve showed good linearty over the glucose concentration of 0.05~1 mmol/L. The limit of detection for the method herein reported was 6.2 μmol/L, with recovery in the range of 76.4%~90.0%, and the RSD was 3.18%.

     

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