陈秀. 27种胺类物质与间甲基苯甲酰氯的衍生化特点及LC-MS定性分析方法[J]. 分析测试技术与仪器, 2016, 22(1): 28-35. DOI: 10.16495/j.1006-3757.2016.01.005
引用本文: 陈秀. 27种胺类物质与间甲基苯甲酰氯的衍生化特点及LC-MS定性分析方法[J]. 分析测试技术与仪器, 2016, 22(1): 28-35. DOI: 10.16495/j.1006-3757.2016.01.005
CHEN Xiu. Derivatization Characteristics of Twenty Seven Kinds of Amines with Methylbenzoyl Chloride and Qualitative Analysis Method by LC-MS[J]. Analysis and Testing Technology and Instruments, 2016, 22(1): 28-35. DOI: 10.16495/j.1006-3757.2016.01.005
Citation: CHEN Xiu. Derivatization Characteristics of Twenty Seven Kinds of Amines with Methylbenzoyl Chloride and Qualitative Analysis Method by LC-MS[J]. Analysis and Testing Technology and Instruments, 2016, 22(1): 28-35. DOI: 10.16495/j.1006-3757.2016.01.005

27种胺类物质与间甲基苯甲酰氯的衍生化特点及LC-MS定性分析方法

Derivatization Characteristics of Twenty Seven Kinds of Amines with Methylbenzoyl Chloride and Qualitative Analysis Method by LC-MS

  • 摘要: 研究了27种胺类物质在与间甲基苯甲酰氯的衍生化过程中, 活泼氢与衍生化试剂的反应情况.建立了超高液相色谱-质谱分析27种胺类衍生物的定性分析方法.使用Waters ACQUITY UPLC®HSS T3色谱柱(Φ1.8 μm, 3.0 mm×150 mm)为分离柱, 乙腈和甲酸(体积比为0.1%)水溶液为流动相, 采用2种梯度洗脱程序, 经液相色谱分离后, 质谱采用电喷雾电离源正离子模式, 以各种胺类衍生化合物的选择离子+监测模式进行定性分析.试验发现, 仪器检出限可达0.2 mg/L, 可以用于胺类物质的快速定性检测.

     

    Abstract: In the process of derivation of twenty seven kinds of amines with methylbenzoyl chloride, the reaction between active hydrogen and derivative reagent was studied. A new method has been developed for the determination of twenty seven amine derivatives by the ultra high liquid chromatography-mass spectrometry. Waters ACQUITY UPLC®HSS T3(Φ1.8 μm, 3.0 mm×150 mm) was employed as the analytical column, the mobile phase were acetonitrile and formic acid (0.1%), two gradient elution procedures were used. After the separation by liquid chromatography, the mass spectrometric qualitative analysis was performed based on the selective ionic measurement M+H+ using the electro-spray ionization in positive mode. The instrument detection limits of amines were 0.2 mg/L. The proposed method was successfully applied to the fast qualitative analysis of amines.

     

/

返回文章
返回