吴娜, 刘卫, 田先娇, 杨新周. 气相色谱-质谱指纹图谱法研究普洱茶醇溶物挥发性成分[J]. 分析测试技术与仪器, 2020, 26(4): 270-276. DOI: 10.16495/j.1006-3757.2020.04.007
引用本文: 吴娜, 刘卫, 田先娇, 杨新周. 气相色谱-质谱指纹图谱法研究普洱茶醇溶物挥发性成分[J]. 分析测试技术与仪器, 2020, 26(4): 270-276. DOI: 10.16495/j.1006-3757.2020.04.007
Na WU, Wei LIU, Xian-jiao TIAN, Xin-zhou YANG. Study on Volatile Components in Alcohol Solute in Pu-erh Tea by Gas Chromatographic-Mass Spectrometric Fingerprint Method[J]. Analysis and Testing Technology and Instruments, 2020, 26(4): 270-276. DOI: 10.16495/j.1006-3757.2020.04.007
Citation: Na WU, Wei LIU, Xian-jiao TIAN, Xin-zhou YANG. Study on Volatile Components in Alcohol Solute in Pu-erh Tea by Gas Chromatographic-Mass Spectrometric Fingerprint Method[J]. Analysis and Testing Technology and Instruments, 2020, 26(4): 270-276. DOI: 10.16495/j.1006-3757.2020.04.007

气相色谱-质谱指纹图谱法研究普洱茶醇溶物挥发性成分

Study on Volatile Components in Alcohol Solute in Pu-erh Tea by Gas Chromatographic-Mass Spectrometric Fingerprint Method

  • 摘要: 以普洱茶7542为参考样本, 乙醇为溶剂, 超声提取制备普洱茶醇溶物, 建立了普洱茶7542醇溶物气相色谱-质谱(GC-MS)指纹图谱, 同时对其指纹图谱进行相似度计算和主成分分析.试验结果表明, 普洱茶醇溶物的最佳提取方法:采用50 mL 95%乙醇超声提取30 min, 顶空进样最佳条件为振荡箱温度110℃, 振荡时间20 min.通过对9个不同年限、不同批次的普洱茶7542系列样品醇溶物挥发性成分GC-MS指纹图谱进行相似度分析, 测定样品醇溶物挥发性成分图谱与对照图谱之间的相似度在0.706~0.906之间, 说明不同年限、不同批次的9个普洱茶7542醇溶物挥发性成分和参照样本相比发生了改变, 但变化较小, 但其共有成分峰面积百分含量存在差异.通过对9个不同年限、不同批次普洱茶7542醇溶物挥发性成分共有成分进行主成分分析, 提取了2个成分, 即2-羟甲基-2-甲基-吡咯烷-1-甲醛和N-丁基苯磺酰胺, 其特征值大于1, 累计方差贡献率达90.2%, 能较好的代表 9个不同年限、不同批次普洱茶7542的全部信息, 可以用主成分分析来反映样品的全部信息.因此可通过测定9个不同年限、不同批次普洱茶7542醇溶物中2-羟甲基-2-甲基-吡咯烷-1-甲醛和N-丁基苯磺酰胺的含量差异评价普洱茶7542的质量.

     

    Abstract: The alcohol-soluble substances were extracted with the ultrasonic extraction using ethanol as the solvent from Pu-erh tea 7542, used as the reference sample.The gas chromatographic-mass spectrometric (GC-MS) fingerprint of alcohol-soluble substances in Pu-erh tea has been established, and the similarity calculation and principal components were analyzed.The results showed that the optimal extraction method of alcohol-soluble substances in Pu-erh tea were as follows:50 mL 95% ethanol was used as the solvent and ultrasonically extracted for 30 min? the optimum headspace oscillator temperature was 110℃ with the oscillation duration for 20 min.The similarity of the volatile constituents GC-MS fingerprint of alcohol-soluble substances in Pu-erh tea and the reference fingerprint was between 0.706 and 0.906, which showed that the volatile constituents had slight differences in the 9 different ages and batches of Pu-erh teas, but the peak area percentages of the common components varied greatly.Through the principal component analysis of the common components of volatile components of the alcohol-soluble substances in 9 different ages and batches of Pu-erh tea 7542, two components, 2-hydroxymethyl-2-methyl-pyrrolidine-1-carbaldehyde and N-butylbenzene sulfonamide extracted.Their eigenvalue were greater than 1 and the contribution rate was 90.2%, which can better reflect the main volatile components in the alcohol solute of Pu-erh tea 7542 series.Therefore, the assay of 2-hydroxymethyl-2-methyl-pyrrolidine-1-carbaldehyde and N-butylbenzene sulfonamide can decide the quality of different ages and batches of Pu-erh teas.

     

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