Abstract: With the rapid development of domestic higher education, the national investment in higher education is increasing year by year, the scope of colleges and universities is becoming larger and larger, and the large-scale scientific instruments purchased by colleges and universities are also increasing. How to purchase large-scale scientific instruments scientifically, and reduce repeated investment and improve the opening rate and utilization rate of large-scale scientific instruments are a thorny problem faced by many colleges and universities in China. In view of the current situation and existing problems of large-scale scientific instruments management in colleges and universities, an open and shared operation mechanism suitable for these instruments in colleges and universities from the aspects of perfecting the system, changing ideas, investing fund, management means and talent team was established, so as to improve the efficiency of large-scale scientific instruments and give full play to the performance of asset use.