范可青, 凌霞. 高效液相色谱-二极管阵列检测器法测定复方丹参片中12种成分及掺伪鉴别[J]. 分析测试技术与仪器, 2022, 28(3): 353-357. DOI: 10.16495/j.1006-3757.2022.03.019
引用本文: 范可青, 凌霞. 高效液相色谱-二极管阵列检测器法测定复方丹参片中12种成分及掺伪鉴别[J]. 分析测试技术与仪器, 2022, 28(3): 353-357. DOI: 10.16495/j.1006-3757.2022.03.019
FAN Ke-qing, LING Xia. Determination of 12 Index Components and Illegally Added Components in Compound Danshen Tablets by High Performance Liquid Chromatography-Diode Array Detector[J]. Analysis and Testing Technology and Instruments, 2022, 28(3): 353-357. DOI: 10.16495/j.1006-3757.2022.03.019
Citation: FAN Ke-qing, LING Xia. Determination of 12 Index Components and Illegally Added Components in Compound Danshen Tablets by High Performance Liquid Chromatography-Diode Array Detector[J]. Analysis and Testing Technology and Instruments, 2022, 28(3): 353-357. DOI: 10.16495/j.1006-3757.2022.03.019

高效液相色谱-二极管阵列检测器法测定复方丹参片中12种成分及掺伪鉴别

Determination of 12 Index Components and Illegally Added Components in Compound Danshen Tablets by High Performance Liquid Chromatography-Diode Array Detector

  • 摘要: 采用高效液相色谱-二极管阵列检测器法测定复方丹参片中12种成分并判定是否掺有三七茎叶. 流动相为乙腈-0.1%磷酸水溶液(梯度洗脱),通过变换波长结合紫外光谱扫描定性的方式对丹参素钠、原儿茶酸、原儿茶醛、迷迭香酸、丹酚酸B、三七皂苷R1、人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1、人参皂苷Rb3、丹参酮I、丹参酮IIA进行分析. 12种成分的线性关系良好(r≥0.999 2),精密度(RSD<2.0%)、稳定性(RSD<2.0%)、回收率(96.1%~99.8%)均符合方法学要求,可用于复方丹参片的质量控制.

     

    Abstract: An analysis method has been established for the determination of 12 components in compound Danshen tablets by high performance liquid chromatography-diode array detector, and it was determined whether there were illegally mixed Panax notoginseng leaves in compound Danshen tablets. The mobile phase was acetonitrile-0.1% aqueous phosphate solution (gradient elution). The sodium Danshensu, protocatechuic acid, protocatechualdehyde, rosmarinic acid, salvianolic acid B, notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rb3, tanshinone I, tanshinone IIA were analyzed by changing the wavelengthes and UV spectral scanning qualitative method. The linear relationship of the 12 components was good (r≥0.999 2), and the precision (RSD<2.0%), stability (RSD<2.0%) and recovery rate (96.1%~99.8%) all met the methodological requirements, which can be used for the quality control of compound Danshen tablets.

     

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