Abstract: The content of chlorogenic acid in honeysuckle was determined by high performance liquid chromatography (HPLC), the extraction conditions of chlorogenic acid from honeysuckle were optimized using a thermal reflux method and the orthogonal test was carried out. The results showed that the best extraction conditions of chlorogenic acid from honeysuckle was ethanol with a volume fraction of 80%, a solid-liquid ratio of 1∶20 (g/mL), an extraction temperature of 100 ℃, an extraction time of 2 h, and the extraction rate of chlorogenic acid was 7.1%. The method can be used for quality control, development and utilization of honeysuckle with accurate analysis, simple operation and low equipment requirements.