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LU Qiao-mei. Sensitive Analysis of Microcystins in Aquatic Products by Nano-Flow Chip Liquid Chromatography-Mass Spectrometry[J]. Analysis and Testing Technology and Instruments, 2019, 25(2): 65-71. doi: 10.16495/j.1006-3757.2019.02.001
Citation: LU Qiao-mei. Sensitive Analysis of Microcystins in Aquatic Products by Nano-Flow Chip Liquid Chromatography-Mass Spectrometry[J]. Analysis and Testing Technology and Instruments, 2019, 25(2): 65-71. doi: 10.16495/j.1006-3757.2019.02.001

Sensitive Analysis of Microcystins in Aquatic Products by Nano-Flow Chip Liquid Chromatography-Mass Spectrometry

doi: 10.16495/j.1006-3757.2019.02.001
  • Received Date: 2019-02-20
  • Rev Recd Date: 2019-03-13
  • Publish Date: 2019-06-30
  • A method for the simultaneous analysis of 3 microcystins (MC-RR, MC-YR and MC-LR) was set up by the nano-flow chip liquid chromatography-high resolution mass spectrometry. Under optimal conditions, the mentioned microcystins were baseline separated in 16 min with an ESI source in the positive ion mode. Good linear range in 2.5~2 000 ng/mL was obtained, with the correlation coefficient larger than 0.996 5 and the limit of detection between 0.5~2.0 ng/mL. Solid phase extraction method was used for the extraction and clean-up of fish and shrimp samples. No residue of the three microcystins was detected and the spiked recoveries were between 82.8%~120.3%. The developed method is sensitive, rapid and exact with low sample consumption, so it is reliable for the trace determination of microcystins in biological samples including aquatic products.
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  • [1]
    赵漫, 李冰, 马燕天, 等.应用环境微生物治理淡水湖泊微囊藻毒素污染的研究进展[J].微生物学通报, 2018, 45(4):893-899. http://d.old.wanfangdata.com.cn/Periodical/wswxtb201804018

    ZHAO Man, LI Bing, MA Yan-Tian, et al. Advances in the application of environmental microbes to control microcystins in freshwater lake[J]. Microbiology China, 2018, 45(4):893-899. http://d.old.wanfangdata.com.cn/Periodical/wswxtb201804018
    [2]
    Pham T L, Utsumi M. An overview of the accumulation of microcystins in aquatic ecosystems[J]. J Environ Manage, 2018, 213:520-529. doi: 10.1016/j.jenvman.2018.01.077
    [3]
    钟力, 徐文琦, 胡静文, 等.微囊藻毒素的危害及其分析方法进展[J].分析化学进展, 2018, 8(3):91-102. http://d.old.wanfangdata.com.cn/Periodical/ahsfdx201401011

    ZHONG Li, XU Wen-qi, HU Jing-wen, et al. The harmful effects of microcystins and the analytical methods[J]. Advances in Analytical Chemistry, 2018, 8(3):91-102. http://d.old.wanfangdata.com.cn/Periodical/ahsfdx201401011
    [4]
    吴伟文, 杨左军, 顾浩飞, 等.固相微萃取/高效液相色谱法测定水中的微囊藻毒素[J].分析测试学报, 2007, 26(4):545-547. doi: 10.3969/j.issn.1004-4957.2007.04.023

    WU Wei-wen, YANG Zuo-jun, GU Hao-fei, et al. Determination of trace microcystin in water by solid phase microextraction coupled with high performance liquid chromatography[J]. Journal of Instrumental Analysis, 2007, 26(4):545-547. doi: 10.3969/j.issn.1004-4957.2007.04.023
    [5]
    郭亭秀, 娄大伟, 连丽丽, 等. Fe3O4@NiSiO3对水中痕量微囊藻毒素的萃取[J].分析测试学报, 2016, 35(9):1137-1141. doi: 10.3969/j.issn.1004-4957.2016.09.011

    GUO Ting-xiu, LOU Da-wei, LIAN Li-li, et al. Extraction of trace mierocystins in water using Fe3O4@NiSiO3[J]. Journal of Instrumen- tal Analysis, 2016, 35(9):1137-1141. doi: 10.3969/j.issn.1004-4957.2016.09.011
    [6]
    Qian Z Y, Li Z G, Ma J, et al. Analysis of trace microcystins in vegetables using matrix solid-phase dispersion followed by high performance liquid chromatography triple-quadrupole mass spectrometry detection[J]. Talanta, 2017, 173:101-106. doi: 10.1016/j.talanta.2017.05.079
    [7]
    Li S Y, Cui Y W, Wang Y, et al. A shotgun method for high throughput screening microcystins in margarya melanioides on a triple quadrupole tandem mass spectrometry[J]. Food Chem, 2018, 269:89-95. doi: 10.1016/j.foodchem.2018.07.004
    [8]
    Haghighi F, Talebpour Z, Nezhad A S. Towards fully integrated liquid chromatography on a chip:evolution and evaluation[J]. TrAC Trends Anal Chem, 2018, 105:302-337. doi: 10.1016/j.trac.2018.05.002
    [9]
    Yin H, Killeen K. The fundamental aspects and applications of agilent HPLC-Chip[J]. J Sep Sci, 2007, 30(10):1427-1434. doi: 10.1002/(ISSN)1615-9314
    [10]
    Li X J, Fekete A, Englmann M, et al. At-line coupling of UPLC to chip-electrospray-FTICR- MS[J]. Anal Bioanal Chem, 2007, 389(5):1439-1446. doi: 10.1007/s00216-007-1524-4
    [11]
    Zhang Y, Li Y Y, Qiu F, et al. Comparative analysis of the human urinary proteome by 1D SDS-PAGE and chip-HPLC-MS/MS identification of the AACT putative urinary biomarker[J]. J Chromatogr B, 2010, 878(32), 3395-3401. doi: 10.1016/j.jchromb.2010.10.026
    [12]
    Kozlik P, Sanda M, Goldman R. Nano reversed phase versus nano hydrophilic interaction liquid chromato- graphy on a chip in the analysis of hemopexin glycopeptides[J]. J Chromatogr A, 2017, 1519(23):152-155.
    [13]
    Min J Z, Yano H, Matsumoto A, et al. Simultaneous determination of polyamines in human nail as 4-(N, N-dimethylaminosulfonyl)-7-fluoro-2, 1, 3-benzoxadiazole derivatives by nano-flow chip LC coupled with quadrupole time-of-flight tandem mass spectrometry[J]. Clin Chim Acta, 2011, 412(1-2):98-106. doi: 10.1016/j.cca.2010.09.018
    [14]
    Zhu K Y, Leung K W, Ting A K L, et al. The establishment of a highly sensitive method in detecting ketamine and norketamine simultaneously in human hairs by HPLC-Chip-MS/MS[J]. Forensic Sci Int, 2011, 208(1-3):53-58. doi: 10.1016/j.forsciint.2010.10.028
    [15]
    Zhao C X, Wu Z M, Xue G, et al. Ultra-high capacity liquid chromatography chip/quadrupole time-of-flight mass spectrometry for pharmaceutical analysis[J]. J Chromatogr A, 2011, 1218(23):3669-3674. doi: 10.1016/j.chroma.2011.04.020
    [16]
    中华人民共和国卫生部. GB 5749-2006生活饮用水卫生标准[S].

    The Minister of Health of the People's Republic of China. GB 5749-2006 Standards for drinking water quality[S].
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