Detection of 4 Fungicides in Water by Solid Phase Extraction- Ultra-High Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

A solid phase extraction-ultra-high performance liquid chromatography-triple quadrupole mass spectrometry method has been established to detect four fungicides (azoxystrobin, tebuconazole, pyrazole ether ester and oxime ester) in water. The fungicides in water samples were enriched on a HLB solid phase extraction column, separated on a C18 chromatographic column, and analyzed by ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS) with an external standard. The detection method was a multiple reaction monitoring (MRM). And the effects of pH, elution solvent, and the amount of elution solvent on the extraction effect were investigated. The experimental quantitative analysis adopted the external standard method. The four fungicides displayed a good linear relationship in the concentration range of 0.10~50.0 μg/L, the detection limit was 0.004~0.095 μg/L, the recoveries were 68.7%~95.4%, and the relative standard deviations (n=5) were less than 10%. Using the method to detect fungicides is simple, fast, accurate, and sensitive.