Determination of 30 Trace Elements in Blood by Microwave Digestion-Inductively Coupled Plasma-Mass Spectrometry

The method of determining 30 trace elements in blood has been developed using the microwave digestion-inductively coupled plasma-mass spectrometry. The samples were pretreated with a nitric acid-hydrogen peroxide mixed system with microwave digestion, and then diluted to be determined by inductively coupled plasma-mass spectrometry (ICP-MS). The contents of copper (Cu), lead (Pb), zinc (Zn), silver (Ag), barium (Ba), tin (Sn), nickel (Ni), cobalt (Co), vanadium (V), antimony (Sb), thallium (Tl), mercury (Hg), manganese (Mn), molybdenum (Mo), selenium (Se), chromium (Cr), cadmium (Cd), thorium (Th), titanium (Ti), aluminum (Al), arsenic (As), iron (Fe), calcium (Ca), gallium (Ga), strontium (Sr), lithium (Li), magnesium (Mg), beryllium (Be), sodium (Na) and potassium (K) in the whole blood were determined. The sample pretreatment process and all the instrument parameters were optimized. The matrix interference effect was reduced by the internal standard correction method. The results indicated that all the determined elements were in a good linear relationship with the response abundance of mass spectrometry in the corresponding concentration range. The detection precision was 0.142%~12.856%. The method has the advantages of simple, accurate and rapid, and is suitable for the accurate determination of the contents of various trace elements in the whole blood.