Study on Extraction Method of Chlorogenic Acid from Honeysuckle

The content of chlorogenic acid in honeysuckle was determined by high performance liquid chromatography (HPLC), the extraction conditions of chlorogenic acid from honeysuckle were optimized using a thermal reflux method and the orthogonal test was carried out. The results showed that the best extraction conditions of chlorogenic acid from honeysuckle was ethanol with a volume fraction of 80%, a solid-liquid ratio of 1∶20 (g/mL), an extraction temperature of 100 ℃, an extraction time of 2 h, and the extraction rate of chlorogenic acid was 7.1%. The method can be used for quality control, development and utilization of honeysuckle with accurate analysis, simple operation and low equipment requirements.