<i>meso</i>-四-[4-(Boc-苏氨酸)氨基苯基]卟啉荧光探针对<i>L</i>-色氨酸的痕量测定

蔡燕燕; 谭卫红; 马艳; 沈娟章; 魏琴

meso-四-[4-(Boc-苏氨酸)氨基苯基]卟啉荧光探针对L-色氨酸的痕量测定

1.
中国林业科学研究院林产化学工业研究所, 生物质化学利用国家工程实验室, 国家林业局林产化学工程重点开放性实验室, 江苏省生物质能源与材料重点实验室, 江苏南京 210042
2.
济南大学化学化工学院, 山东济南 250022

基金项目:

国家自然科学基金（20577016）和山东省自然科学基金（Y2008B44）

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- 被引次数: 0
出版历程
- 收稿日期: 2013-09-24
- 修回日期: 2013-10-21
- 刊出日期: 2013-12-27

Determination of Trace Amounts of L-tryptophan Using Tetra-[(4-Bocthreonine)-aminophenyl]Porphyrin as Fluorescence Spectral Probe

1.
Institute of Chemical Industry of Forestry Products, CAF, Key Laboratory of Biomass Energy and Material, Jiangsu Province, National Engineering Laboratory for Biomass Chemical Utilization, Key and Laboratory on Forest Chemical Engineering, SFA, Nanjing 210042, China
2.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China

摘要

摘要: 利用荧光光谱方法探讨了以对称型阳离子氨基酸卟啉：meso-四-[4-(Boc-苏氨酸)氨基苯基]卟啉（TAPP-Thr-Boc）作为光谱探针测定L-色氨酸的最佳条件. 实验结果表明：双-2-乙基己基硫代琥珀酸钠（AOT）表面活性剂的加入能显著增加体系的灵敏度. 在最佳实验条件下，体系荧光强度的下降程度与L-色氨酸含量在0.10~8.80 μg/mL范围内呈线性关系，检出限为0.034 μg/mL. 方法抗干扰能力强，有较好的选择性和灵敏度，用于实际样品仔猪饲料的测定，结果满意.
- 氨基酸卟啉 /
- L-色氨酸 /
- 荧光光谱法 /
- 荧光探针
Abstract: The optimum experimental conditions for the determination of L-tryptophan were investigated by fluorescence spectrophotometry using tetra-[(4-Bocthreonine)-aminophenyl]porphyrin as the fluorescence spectral probe. Experiments indicated that the sensitivity of the system can be enhanced significantly by the addition of bis(2-ethylhexyl)sulfosuccinate sodium salt (AOT). Under optimum experimental conditions, the decreased of the fluorescence intensity of the system was in proportion to the concentration of L-tryptophan in the concentration range of 0.10~8.80 μg/mL with a limit of detection of 0.034 μg/mL. The method has been used in the determination of samples with satisfactory results.
- amino-acid porphyrin /
- L-tryptophan /
- fluorescent spectrometry /
- fluorescent probe

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参考文献(16)

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