E-alert
Ultrasonic-Assisted Extraction of Anthocyanins from RubusCorchorifolius L. f and Antioxidant Activity
-
摘要: 在单因素试验的基础上选择提取次数、提取试剂、料液比、超声时间4个因素为自变量，以树莓花色苷的提取率为响应值，进行Box-Behnken中心组合试验设计，采用响应面法（RSM）评估了这些因素对花色苷提取率的影响.并分别采用1，1-二苯基苦基苯肼（DPPH）和2，2-联氮-二（3-乙基-苯并噻唑-6-磺酸）二铵盐（ABTS）法对花色苷的抗氧化活性进行研究.结果表明，超声辅助提取树莓中花色苷的最佳工艺条件为：超声提取3次，料液比1:46 g/mL，超声时间44 min，在此条件下预测花色苷的得率为6.79%.花色苷对于DPPH的清除率达到13.68 μmol/g，对于ABTS的清除率达到8.37 μmol/g.Abstract: Based on single factor experiments, extraction times, extraction reagent, solid-liquid ratio, ultrasonic time were selected for the Box-Behnken central composite design. Response surface method was employed to study the effect of these factors on the yield of anthocyanins and the antioxidant activity of anthocyanins. 2, 2-diphenyl-1-picrylhydrazyl(DPPH) and 2, 2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)method were used, respectively to study the antioxidant activity of anthocyanins in raspberry. The results indicated that optimal conditions were:ultrasonic extraction for 3 times, 1:46 g/mL as the ratio of liquid to solid with 44 min extraction. Under these optimized conditions, the extraction yield of anthocyanins was up to 6.79%. The clearance of DPPH was 13.68 μmol/g and the clearance of ABTS was 8.37 μmol/g. The study provides scientific and theoretical basis for the research and utilization of RubusCorchorifolius L. f.
-
Key words:
- RubusCorchorifolius L. f /
- anthocyanins /
- response surface method /
- antioxidant
-
图 1 超声次数对花色苷得率的影响
Figure 1. Effect of extraction times on extraction yield of anthocyanins
图 2 料液比对花色苷得率的影响
Figure 2. Effect of ratio of solid to liquid on extraction yield of anthocyanins
图 3 提取试剂对花色苷得率的影响
Figure 3. Effect of extracting agent on extraction yield of anthocyanins
图 4 超声时间对花色苷得率的影响
Figure 4. Effect of ultrasonic temperature on extraction yield of anthocyanins
图 5 各两因素交互作用对花色苷提取率影响的响应面图
Figure 5. Response surface plots of mutual influences of extraction conditions on extraction yields of anthocyanins
表 1 中心组合设计因素与水平表
Table 1. Factors and levels in central composite design
因素 水平 -1 0 1 提取次数(A) 2 3 4 料液比(B) 1:30 1:40 1:50 提取时间(C)/ ℃ 30 40 50 
下载: 导出CSV
表 2 拟合二次多项式模型的方差分析
Table 2. Analysis of variance(ANOVA)for fitted quadratic polynomial model
方差来源 平方和 自由度 均方 F值 P值 显著性 Model 66.50 9 66.50 187.19 ＜0.000 1 Siginicant A 1.31 1 1.31 33.24 0.000 7 B 0.15 1 0.15 3.83 0.091 2 C 6.81 1 6.81 172.48 ＜0.000 1 AB 0.32 1 0.32 8.23 0.024 0 AC 0.029 1 0.029 0.73 0.420 5 BC 0.66 1 0.66 16.62 0.004 7 A2 21.84 1 21.84 553.31 ＜0.000 1 B2 23.10 1 23.10 585.35 ＜0.000 1 C2 6.71 1 6.71 170.03 ＜0.000 1 残差 0.28 7 0.039 失逆性 0.21 3 0.071 4.40 0.093 0 Not siginicant 纯误差 0.064 4 0.016 总差 66.77 16 注：P＜0.01，差异极显著；P＜0.05，差异显著. R2=0.993 8
下载: 导出CSV
-
[1] 中国科学院西北高原生物研究所.青海植物志[M].西宁:青海人民出版社, 1997:393. [2] 韩加, 新华·纳比, 阿里木·帕塔尔, 等.新疆树莓果实营养成分及其提取物抗氧化性研究[J].营养学报, 2008, 30(4):410-413. http://www.cnki.com.cn/Article/CJFDTOTAL-YYXX200804024.htm [3] 刘孟军.中国野生果树[M].北京:中国农业出版社, 1998:227-231. [4] 刘建华, 张志军, 李淑芳.树莓中功效成分的开发浅论[J].食品科学, 2004, 25(10):370-373. doi: 10.3321/j.issn:1002-6630.2004.10.092 [5] Mong Liu, Xinqi Li. Antioxidant and antiprolife -rative activities of raspberries[J]. J Agric Food Chem, 2002, 50:2926-2930. doi: 10.1021/jf0111209 [6] ETIN EKIC, MUSTAFA ZGEN. Comparison of antioxidant capacity and phytochemical properties of wild and cultivated red raspberries(Rubusidaeus L.)[J]. Journal of Food Composition and Analysis, 2009(10):1-21. https://www.researchgate.net/publication/248579897_Comparison_of_antioxidant_capacity_and_phytochemical_properties_of_wild_and_cultivated_red_raspberries_Rubus_idaeus_L [7] CHEN Fang, SUN Yangzhao, ZHAO Guanghua, et al. Optimization of ultrasoundassisted extraction of anthocyanins in red raspberries and identification of anthocyanins in extract using high-performance liquid chromatography-mass spectrometry[J]. Ultrasonics Sonochemistry, 2007(14):767-778. https://www.researchgate.net/publication/6484092_Optimization_of_ultrasound-assisted_extraction_of_anthocyanins_in_red_raspberries_and_identification_of_anthocyanins_in_extract_using_high-performance_liquid_chromatography-mass_spectrometry [8] 李传欣, 张华, 李景琳.食用天然色素的应用及发展趋势[J].辽宁农业科学, 2001(1):29-32. http://www.cnki.com.cn/Article/CJFDTOTAL-GSKJ200305023.htm [9] 徐俐, 金毅.树莓红色素的提取及稳定性的研究[J].食品科学, 2006, 27(5):191-194. http://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ200712058.htm [10] 白立敏, 辛秀兰.树莓红色泰的提取与稳定性研究[J].食品工业科技, 2007, 28(12):179-195. doi: 10.3969/j.issn.1002-0306.2007.12.056 [11] 石岳, 张秀玲, 邹阳.pH示差法测定黑莓残渣中花青素含量的研究[J].中国食品工业, 2007, 12(24):56-57. http://www.cnki.com.cn/Article/CJFDTOTAL-SPZG200712023.htm [12] 冯建光, 谷之英.葡萄皮色素的示差法测定[J].分析检测, 2001, 9(1):85-86. http://kns.cnki.net/KCMS/detail/detail.aspx?filename=spkj200209042&dbname=CJFD&dbcode=CJFQ [13] 王煜伟, 杨永晶, 胡娜, 等.响应面法优化树莓中总黄酮的超声提取工艺研究[J].天然产物研究与开发, 2014, 26(1):128-132. http://www.cnki.com.cn/Article/CJFDTOTAL-XBLX201301023.htm [14] 王煜伟, 杨永晶, 胡娜, 等.响应面法优化树莓中总黄酮的超声提取工艺研究[J].食品工业, 2015, 220(1):67-70. http://www.cnki.com.cn/Article/CJFDTOTAL-SPGY201501020.htm [15] LUO W, ZHAO M M, YANG B, et al. Identification of bioactive compounds in Phyllenthusemblica L. fruit and their free radical scavengingactivities[J]. Food Chemistry, 2009, 114:499-504. doi: 10.1016/j.foodchem.2008.09.077 [16] RE R, PELLEGRINI N, PROTEGGENTE A, et al. Antioxidant activity applying an improved ABTS radical cation decolorization assay[J].Free Radical Biology & Medicine, 1999, 26:1231-1237. http://www.sigmaaldrich.com/catalog/papers/10381194 [17] 王睿婷, 王鑫, 张富敏, 等.树莓的抗氧化活性与主要化学成分的相关分析[J].沈阳药科大学学报, 2013(11):901-905. http://www.cnki.com.cn/Article/CJFDTOTAL-SYYD201311017.htm [18] 吴春.花色素抗氧化作用的研究[J].哈尔滨商业大学学报, 2003, 4(3):492-494. http://cdmd.cnki.com.cn/Article/CDMD-10335-1016042621.htm [19] Mc CORD J M. Oxygen-derived free radicals in postischemic tissue injury[J]. New England Journal of Medicine, 1985, 312:159-163. doi: 10.1056/NEJM198501173120305 [20] 肖军霞, 黄国清, 仇宏伟, 等.红树莓花色苷的提取及抗氧化活性研究[J].食品科学, 2011, 32(8):15-18. http://www.cnki.com.cn/Article/CJFDTOTAL-SPKX201108003.htm -
点击查看大图
计量
- 文章访问数: 1142
- HTML全文浏览量: 73
- PDF下载量: 24
- 被引次数: 0
