高效液相色谱法同时测定染发剂中10种染料组分

王海瑞; 陈少波; 徐振东

doi:10.16495/j.1006-3757.2018.01.005

高效液相色谱法同时测定染发剂中10种染料组分

doi: 10.16495/j.1006-3757.2018.01.005

王海瑞^{1, 2,},
陈少波^{1, 2},
徐振东¹

1.
苏州出入境检验检疫局检验检疫综合技术中心, 江苏苏州 215104
2.
苏州世标检测技术有限公司, 江苏苏州 215104

基金项目:

江苏检验检疫局科技计划项目 2017KJ48

详细信息

作者简介:
王海瑞, 女, 硕士研究生, 工程师, 研究方向:化妆品理化分析研究, E-mail:475824201@qq.com

中图分类号: O657.7
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- 被引次数: 0
出版历程
- 收稿日期: 2017-12-05
- 修回日期: 2018-03-12
- 刊出日期: 2018-03-01

Simultaneous Determination of 10 Dye Components in Hair Dye by High Performance Liquid Chromatography

WANG Hai-rui^{1, 2
,},
CHEN Shao-bo^{1, 2},
XU Zhen-dong¹

1.
Comprehensive Technology Center of Suzhou Entry and Exit Inspection and Quarantine Bureau, Suzhou 215104 Jiangsu China
2.
Suzhou World Standard Testing Technology Co Ltd., Suzhou 215104, Jiangsu China

摘要

摘要: 建立了一种高效液相色谱法同时测定染发剂中对苯二胺等10种氧化型染料组分的检测方法.选用色谱柱Poroshell 120 Bonus-RP，针对对苯二胺、间苯二酚、对氨基苯酚、间氨基苯酚、4-氨基间甲酚、氢醌、4-氨基-2-羟基甲苯、2-硝基对苯二胺、甲苯-2，5-二胺硫酸盐和6-羟基吲哚等10种染料组分进行分析测定.方法在0.01~0.50 mg/mL质量浓度范围内线性相关系数为0.999 86~0.999 96，RSD小于5.91%，回收率为87.2%~106.7%.方法具有简单、快捷、分离效果好等特点.
- 染发剂 /
- 染料 /
- 高效液相色谱法
Abstract: In this article a simultaneous analytical method for the detection of P-phenylene diamine and other 10 oxidation type dye components in hair dye by high performance liquid chromatography is described. The objectives of dye derivatives p-phenylenediamine, resorcine, p-aminopheno, m-aminophenol, 4-amino-m-cresol, hydroquinone, 4-amino-2-hydroxytoluene, p-diamino-2-nitrobenzene, 2, 5-diaminotoluene sulfate, 6-hydroxyindole were seperated on a Poroshell 120 Bonus-RP capillary column. Mass spectrometry was used to quantify the dye in hair dye. Results showed that correlation coefficients of the method were from 0.999 85 to 0.999 96. The relative standard deviation(RSD) was less than 5.91% when the concentrations were in the range of 0.01~0.5 mg/mL, the recovery was from 87.2% to 106.7%. The determination results demonstrated that the method had the feature of simple, rapid, and higly selective.
- hair dye /
- dye /
- high performance liquid chromatography

HTML全文

图 1 染料标准溶液液相色谱图

(1)对苯二胺，(2)间苯二酚，(3)对氨基苯酚，(4)间氨基苯酚，(5) 4-氨基间甲酚，(6)氢醌，(7) 4-氨基-2-羟基甲苯，(8) 2-硝基对苯二胺，(9)甲苯-2, 5-二胺硫酸盐，(10) 6-羟基吲哚

Figure 1. Liquid chromatogram of dye standard soloution

(1) p-phenylenediamine, (2) Resorcine, (3) p-Aminophenol, (4) m-Aminophenol, (5) 4-Amino-m-cresol, (6) Hydroquinone, (7) 4-Amino-2-hydroxytoluene, (8) p-Diamino-2-nitrobenzene, (9) 2, 5-Diaminotoluene sulfate, (10) 6-Hydroxyindole

下载: 全尺寸图片幻灯片

图 2 某样品染发剂中液相色谱图

Figure 2. Liquid chromatogram of Some sample hair dye

下载: 全尺寸图片幻灯片

表 1 洗脱条件

Table 1. Elution conditions

时间/min	流动相A(10 mmol/L乙酸铵)/%	B(乙腈)/%
0	95	5
2.0	95	5
8.0	80	20
10.0	10	90
12.5	10	90
13.0	95	5
16.0	95	5

下载: 导出CSV

表 2 线性方程、相关系数与检出限

Table 2. Liner equations, correlation coefficients and detection limits

染料组分	线性方程	线性相关系数(R)	检出限/(mg/L)
对苯二胺	Y=4.07X-0.60	0.9999 1	8.0
间苯二酚	Y=2.27X+0.99	0.9999 0	5.0
对氨基苯酚	Y=6.44X=2.41	0.9998 6	8.0
间氨基苯酚	Y=3.98X+1.71	0.9999 0	5.0
4-氨基间甲酚	Y=4.06X+0.04	0.9999 2	8.0
氢醌	Y=4.59X+1.16	0.9999 3	6.0
4-氨基-2-羟基甲苯	Y=2.60X+0.24	0.9999 2	6.0
2-硝基对苯二胺	Y=6.26X+2.29	0.9999 4	10.0
甲苯-2, 5-二胺硫酸盐	Y=4.55X-0.35	0.9999 1	3.0
6-羟基吲哚	Y=3.28X+1.24	0.9999 6	4.0

下载: 导出CSV

表 3 染发剂中染料3个添加水平下染料组分加标回收率和相对标准偏差(n=6)

Table 3. Spiked recoveries and RSDs of dye components in hair dye(n=6)

染料组分	加标质量/μg	本底质量/μg	回收质量/μg	回收率/%	RSD/%
对苯二胺	625.0	1 705.0	667.5	106.7	2.21
	1 250.0		1 252.5	100.2	1.46
	2 500.0		2 442.5	97.7	5.05
间苯二酚	625.0	1 453.0	560.0	89.6	3.24
	1 250.0		1 180.0	94.3	1.05
	2 500.0		2 490.0	99.6	1.13
对氨基苯酚	625.0	/	572.5	91.6	5.91
	1 250.0		1 100.0	87.9	2.33
	2 500.0		2 340.0	93.6	3.64
间氨基苯酚	625.0	/	642.5	102.8	1.06
	1 250.0		1 192.5	95.3	1.16
	2 500.0		2 435.0	97.4	2.69
4-氨基间甲酚	625.0	/	612.5	97.9	1.44
	1 250.0		1 322.5	105.8	2.09
	2 500.0		2 217.5	88.7	1.12
氢醌	625.0	/	577.5	92.5	2.94
	1 250.0		1 182.5	94.6	0.87
	2 500.0		2 260.0	90.4	1.08
4-氨基-2-羟基甲苯	625.0	/	617.5	98.9	1.41
	1 250.0		1 195.0	95.6	3.18
	2 500.0		2 407.5	96.3	2.94
2-硝基对苯二胺	625.0	/	545.0	87.2	2.90
	1 250.0		1 145.0	91.6	3.81
	2 500.0		2 367.5	94.7	1.13
甲苯-2, 5-二胺硫酸盐	625.0	2 925.0	579.5	95.4	1.57
	1 250.0		1 120.0	89.6	2.67
	2 500.0		2 330.0	93.2	3.12
6-羟基吲哚	625.0	/	590.0	94.5	2.83
	1 250.0		1 140.0	91.2	3.71
	2 500.0		2 537.5	101.5	2.67

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