Abstract: The distribution, accumulation and elimination of 5 benzene series in mussel tissues were studied using the gas chromatography tandem mass spectrometry (GC-MS/MS). The results showed that there were some differences in the bioaccumulation of 5 benzene series in different tissues of mussel, and the distribution was tissue specific. At the end of the exposure experiment, the distribution and the trend of variation in content the five benzene series in the six tissues of mussels were basically the same. The content of benzene series in digestive glands and gonads increased faster than in other tissues, and the peaks values in the digestive gland and gonad were higher than those in the other four tissues. The contents of benzene series in the digestive gland and gonad were relatively higher, which had obvious absorption and enrichment effect. The contents of 5 benzene series in foot, gill, adductor and mantle were relatively lower, about 20% of that in the digestive gland. The contents of 5 benzene series in mussel tissues during elimination stage were consistent with the first-order nonlinear attenuation model, with correlation coefficients ranging from 0.994 8 to 0.999 9. The purification rates of benzene, toluene, m-xylene, p-xylene and o-xylene of mussels were 98.4%~99.2%, 97.3%~98.8%, 86.1%~95.8%, 90.8%~97.0% and 92.1%~96.6% respectively after 1 day of purification in the exposed group. After 5 days of purification, although the content of benzene series in the tissues of the exposed group tended to be at the level of blank group, but there were still some residues.