基于气相色谱三重四极杆质谱法分析5种苯系物在贻贝体内的分布、蓄积和消除规律

晁铎源; 宋立玲; 王红光; 丁顒; 邵宏宏; 周秀锦

doi:10.16495/j.1006-3757.2023.01.011

基于气相色谱三重四极杆质谱法分析5种苯系物在贻贝体内的分布、蓄积和消除规律

doi: 10.16495/j.1006-3757.2023.01.011

1.
舟山海关综合技术服务中心，浙江舟山　316000
2.
浙江省海洋生态环境监测中心，浙江舟山　316000

基金项目: 浙江省基础公益项目（LGN19C190002），舟山市公益项目（2019C31045）

详细信息

作者简介:
晁铎源（1986−），女，本科，工程师，研究方向：食品质量安全与控制，E-mail：642713790@qq.com

通讯作者:
周秀锦（1974−），女，硕士，高级工程师，研究方向：食品质量安全与控制，E-mail：68261222@qq.com

中图分类号: O657. 63
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- 文章访问数: 111
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- 被引次数: 0
出版历程
- 收稿日期: 2023-01-31
- 录用日期: 2023-03-10
- 修回日期: 2023-03-10
- 刊出日期: 2023-03-31

Study of Distribution, Accumulation and Elimination of 5 Benzene Series in Mussels Using Gas Chromatography Tandem Mass Spectrometry

1.
Zhoushan Customs Comprehensive Technical Service Center, Zhoushan, 316000, Zhejiang China
2.
Zhejiang Marine Ecology and Environment Monitoring Center, Zhoushan, 316000, Zhejiang China

Funds: Basic Public Welfare Project of Zhejiang Province (LGN19C190002), Public Welfare Project in Zhoushan City (2019C31045)

摘要

摘要: 采用气相色谱三重四极杆质谱（GC-MS/MS）技术，研究了5种苯系物暴露后在贻贝各组织中的分布、蓄积和消除规律. 结果表明，贻贝不同组织中对5种苯系物的生物蓄积具有一定的差异，且分布呈组织特异性. 暴露试验结束后，贻贝各组织中5种苯系物分布及其含量变化趋势基本相同，消化腺和性腺中的苯系物含量增加比其他组织快，且消化腺和性腺中的峰值比其他4种组织中的峰值较高. 消化腺和性腺中的苯系物含量相对较高，有明显的吸收和富集作用. 足、鳃、闭壳肌和外套膜中5种苯系物的含量相对较低，约为消化腺中的20%左右. 消除阶段贻贝各组织中5种苯系物的含量符合一阶非线性衰减模型, 相关系数范围为0.9948~0.999 9. 暴露组净化1 d 后，贻贝对苯、甲苯、间二甲苯、对二甲苯和邻二甲苯的净化率范围分别为98.4%~99.2%、97.3%~98.8%、86.1%~95.8%、90.8%~97.0%和92.1%~96.6%. 净化5 d后，暴露组贻贝各组织中苯系物的含量虽趋于空白组水平，但仍有一定的残留.
- 气相色谱三重四极杆质谱 /
- 苯系物 /
- 贻贝 /
- 分布 /
- 累积 /
- 消除
Abstract: The distribution, accumulation and elimination of 5 benzene series in mussel tissues were studied using the gas chromatography tandem mass spectrometry (GC-MS/MS). The results showed that there were some differences in the bioaccumulation of 5 benzene series in different tissues of mussel, and the distribution was tissue specific. At the end of the exposure experiment, the distribution and the trend of variation in content the five benzene series in the six tissues of mussels were basically the same. The content of benzene series in digestive glands and gonads increased faster than in other tissues, and the peaks values in the digestive gland and gonad were higher than those in the other four tissues. The contents of benzene series in the digestive gland and gonad were relatively higher, which had obvious absorption and enrichment effect. The contents of 5 benzene series in foot, gill, adductor and mantle were relatively lower, about 20% of that in the digestive gland. The contents of 5 benzene series in mussel tissues during elimination stage were consistent with the first-order nonlinear attenuation model, with correlation coefficients ranging from 0.994 8 to 0.999 9. The purification rates of benzene, toluene, m-xylene, p-xylene and o-xylene of mussels were 98.4%~99.2%, 97.3%~98.8%, 86.1%~95.8%, 90.8%~97.0% and 92.1%~96.6% respectively after 1 day of purification in the exposed group. After 5 days of purification, although the content of benzene series in the tissues of the exposed group tended to be at the level of blank group, but there were still some residues.
- GC-MS/MS /
- benzene series /
- mussel /
- distribution /
- accumulation /
- elimination

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图 1 5种苯系物在贻贝各组织中的含量变化

Figure 1. Variations of contents of 5 benzene series in mussel tissues

下载: 全尺寸图片幻灯片

图 2 对二甲苯在贻贝不同组织中的蓄积和消除动态变化

Figure 2. Accumulation and depuration characteristics of p-xylene in mussel tissues

下载: 全尺寸图片幻灯片

图 3 对二甲苯在贻贝各组织中的拟合消除模型图

Figure 3. Fitting elimination models of p-xylene in mussel tissues

下载: 全尺寸图片幻灯片

表 1 5种苯系物的保留时间和MRM质谱参数

Table 1. Retention times and MRM mass parameters of 5 benzene series

化合物	保留时间/ min	离子对/（m/z）	碰撞能量/eV	扫描时间/ms
苯	6.35	78.00>52.00^*	15	30
苯	6.35	77.00>51.00	12	30
甲苯	8.67	91.00>65.00^*	12	30
甲苯	8.67	92.00>65.00	27	30
对二甲苯	11.26	106.00>76.00	24	20
对二甲苯	11.26	106.00>91.00^*	12	20
间二甲苯	11.45	106.00>77.00^*	30	20
间二甲苯	11.45	106.00>91.00	18	20
邻二甲苯	12.73	106.00>63.00^*	45	20
邻二甲苯	12.73	106.00>91.00	12	20
*：定量离子对

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表 2 5种苯系物在贻贝各组织中拟合所得的消除速率常数（K_d）和半衰期（t_1/2）

Table 2. Elimination rate constant (K_d) and half-life (t_1/2) obtained by fitting 5 benzene series in mussel tissues

化合物	组织	t_1/2	K_d
苯	足	0.14	4.92
	闭壳肌	0.14	4.98
	外套膜	0.15	4.52
	腮	0.16	5.44
	性腺	0.15	4.51
	消化腺	0.16	4.45
甲苯	足	0.14	4.91
	闭壳肌	0.15	4.74
	外套膜	0.15	4.49
	腮	0.13	5.34
	性腺	0.17	3.98
	消化腺	0.17	4.09
间二甲苯	足	0.18	3.82
	闭壳肌	0.31	2.27
	外套膜	0.31	2.27
	腮	0.19	4.70
	性腺	0.25	2.72
	消化腺	0.21	3.27
对二甲苯	足	0.13	4.23
	闭壳肌	0.26	2.71
	外套膜	0.27	2.59
	腮	0.22	4.19
	性腺	0.26	2.66
	消化腺	0.19	3.66
邻二甲苯	足	0.15	4.64
	闭壳肌	0.17	4.18
	外套膜	0.26	2.69
	腮	0.18	4.83
	性腺	0.22	3.22
	消化腺	0.18	3.91

下载: 导出CSV

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