Ultrasonic-Assisted Extraction of Anthocyanins from RubusCorchorifolius L. f and Antioxidant Activity

Based on single factor experiments, extraction times, extraction reagent, solid-liquid ratio, ultrasonic time were selected for the Box-Behnken central composite design. Response surface method was employed to study the effect of these factors on the yield of anthocyanins and the antioxidant activity of anthocyanins. 2, 2-diphenyl-1-picrylhydrazyl(DPPH) and 2, 2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)method were used, respectively to study the antioxidant activity of anthocyanins in raspberry. The results indicated that optimal conditions were:ultrasonic extraction for 3 times, 1:46 g/mL as the ratio of liquid to solid with 44 min extraction. Under these optimized conditions, the extraction yield of anthocyanins was up to 6.79%. The clearance of DPPH was 13.68 μmol/g and the clearance of ABTS was 8.37 μmol/g. The study provides scientific and theoretical basis for the research and utilization of RubusCorchorifolius L. f.