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LU Qiao-mei. Sensitive Analysis of Microcystins in Aquatic Products by Nano-Flow Chip Liquid Chromatography-Mass Spectrometry[J]. Analysis and Testing Technology and Instruments, 2019, 25(2): 65-71. doi: 10.16495/j.1006-3757.2019.02.001
Citation: LU Qiao-mei. Sensitive Analysis of Microcystins in Aquatic Products by Nano-Flow Chip Liquid Chromatography-Mass Spectrometry[J]. Analysis and Testing Technology and Instruments, 2019, 25(2): 65-71. doi: 10.16495/j.1006-3757.2019.02.001

Sensitive Analysis of Microcystins in Aquatic Products by Nano-Flow Chip Liquid Chromatography-Mass Spectrometry

doi: 10.16495/j.1006-3757.2019.02.001
  • Received Date: 2019-02-20
  • Rev Recd Date: 2019-03-13
  • Publish Date: 2019-06-30
  • A method for the simultaneous analysis of 3 microcystins (MC-RR, MC-YR and MC-LR) was set up by the nano-flow chip liquid chromatography-high resolution mass spectrometry. Under optimal conditions, the mentioned microcystins were baseline separated in 16 min with an ESI source in the positive ion mode. Good linear range in 2.5~2 000 ng/mL was obtained, with the correlation coefficient larger than 0.996 5 and the limit of detection between 0.5~2.0 ng/mL. Solid phase extraction method was used for the extraction and clean-up of fish and shrimp samples. No residue of the three microcystins was detected and the spiked recoveries were between 82.8%~120.3%. The developed method is sensitive, rapid and exact with low sample consumption, so it is reliable for the trace determination of microcystins in biological samples including aquatic products.
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