Determination of 7 Artificial Sweeteners in Drink Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

LIU Tong; YANG You-you; WANG Hao; MIAO Yu-tian; YANG Yong-tan

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Article Navigation > Analysis and Testing Technology and Instruments > 2015 > (4): 218-224

LIU Tong, YANG You-you, WANG Hao, MIAO Yu-tian, YANG Yong-tan. Determination of 7 Artificial Sweeteners in Drink Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Analysis and Testing Technology and Instruments, 2015, (4): 218-224.

Citation:

LIU Tong, YANG You-you, WANG Hao, MIAO Yu-tian, YANG Yong-tan. Determination of 7 Artificial Sweeteners in Drink Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Analysis and Testing Technology and Instruments, 2015, (4): 218-224.

Citation:

LIU Tong, YANG You-you, WANG Hao, MIAO Yu-tian, YANG Yong-tan. Determination of 7 Artificial Sweeteners in Drink Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Analysis and Testing Technology and Instruments, 2015, (4): 218-224.

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Determination of 7 Artificial Sweeteners in Drink Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

COFCO Nutrition and Health Research Institute, Beijing Key Laboratory of Nutrition Health and Food Safety, Beijing 102009, China

Received Date: 2015-10-16
Rev Recd Date: 2015-11-24
Publish Date: 2015-12-25

Abstract

Abstract

The method for determination of 7sweeteners in wine, grape wine and juice has been developed using the ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS) with ammonium acetate solution/methanol as the mobile phase. Prior to centrifuge with 13000 r/min for 10 min, samples were diluted. Under optimized conditions, the limits of detection(LOD, S/N=3) were from 0.003 to 8μg/L and the linear range was between 10 and 500μg/L with R² ≥ 0.9981. Recoveries were in the range of 77.10%~118.94%, and RSDs of the peak areas were between 0.19% and 5.24%. This method is simple, sensitive and reliable, and is suitable for the rapid detection of artificial sweeteners in these three drinks.
- UPLC-MS/MS,
- drink,
- artificial sweeteners