Abstract: Zearalenone, a secondary metabolite with estrogenic properties, is produced by several Fusarium species that colonize cereal grains. ZEN is hepatotoxic, immunotoxic and genotoxic. It are contaminates foods and feeds wide rangingly, endangers agriculture and pasturage development, and has become a focused point in the field of food safety. In order to effectively control the mycotoxic contamination on grains and grain byproducts, to develop reliable and sensitive determination methods of zearalenone is necessary. In this paper, current laboratory methods to detect ZEN are reviewed.