To determine accurately the content of cyclic adenosine monophosphate (cAMP) by high performance liquid chromatography(HPLC) in jujube in northern China, Hibar ODS-C18 (250 mm×4.6 mm, 5 μm) was used as the chromatographic column, the mobile phase was methanol:2% acetic acid (volume ratio:5:95), the flow rate was 0.5 mL/min, and the detector was a Waters 996. The detection temperature was 25℃ and the detection wavelength was 254 nm. The standard curve was of good linearity in the range of 0.1~1.0 mg/mL. The recovery rate was 102.1% and the relative standard error was 0.63%. The conditions used in this method can completely separate cAMP and other components in jujube. We found that the contents of cAMP in jujube from different producing areas were different, and the content of cAMP in jujube from Xinjiang was the highest and reached 372 μg/g. This method can accurately determine the content of adenosine phosphate in jujube in northern China, with high sensitivity, thus provides an important technical basis for the extraction and high-value utilization of cAMP in jujube.