离子色谱法在中药化学成分分析中的应用进展

颉东妹; 魏晗婷; 王宁丽; 刘建飞; 郭玫; 邸多隆

doi:10.16495/j.1006-3757.2022.02.012

离子色谱法在中药化学成分分析中的应用进展

doi: 10.16495/j.1006-3757.2022.02.012

颉东妹^{1, 2,},
魏晗婷^{1, 2},
王宁丽²,
刘建飞²,
郭玫^1, ,,
邸多隆^{1, 2, ,}

1.
甘肃中医药大学药学院, 甘肃兰州 730000
2.
中国科学院兰州化学物理研究所, 中国科学院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室, 甘肃兰州 730000

基金项目:

甘肃省重点研发计划 2021-0202-NCC-0163

甘肃省青年科技基金计划 20JR10RA054

甘肃省科技计划项目 21YF5NA135

甘肃省科技计划项目 21CX6NF234

详细信息

作者简介:
颉东妹(1996-), 女, 硕士研究生, 研究方向: 中药学, E-mail: 416174332@qq.com

通讯作者:
郭玫，女，教授，研究方向：中药药效物质基础与质量标准研究，E-mail: guomei@gszy.edu.com

邸多隆，男，研究员，《分析测试技术与仪器》副主编，研究方向：分析化学，E-mail: didl@licp.cas.cn

中图分类号: O657.7⁺5;R284.1
计量
- 文章访问数: 204
- HTML全文浏览量: 79
- PDF下载量: 22
- 被引次数: 0
出版历程
- 收稿日期: 2022-03-03
- 修回日期: 2022-04-14
- 网络出版日期: 2022-07-07
- 刊出日期: 2022-06-30

Application Progress of Ion Chromatography in Analysis of Chemical Components of Traditional Chinese Medicine

XIE Dong-mei^{1, 2
,},
WEI Han-ting^{1, 2},
WANG Ning-li²,
LIU Jian-fei²,
GUO Mei^{1
, ,},
DI Duo-long^{1, 2
, ,}

1.
School of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China
2.
Chinese Academy of Sciences Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China

Funds:

Key Research and Development Program of Gansu Province 2021-0202-NCC-0163

Gansu Province Youth Science and Technology Fund program 20JR10RA054

Science and Technology Plan Project of Gansu Province 21YF5NA135

Science and Technology Plan Project of Gansu Province 21CX6NF234

摘要

摘要: 离子色谱作为一种新型高效液相色谱技术, 最初主要应用于测定样品中的阴阳离子含量, 如今发展为应用于有机酸、生物胺、糖类等化合物的组成及含量测定. 离子色谱法具有操作简便、灵敏度高以及选择性好等优点, 因此, 目前离子色谱法已在能源、环境、地质、食品、药物等领域得到了广泛应用. 总结了近年来离子色谱在中药金属离子、无机阴离子、有机酸、糖类成分的研究进展, 为离子色谱法分析中药化学成分提供了参考.
- 中药 /
- 离子色谱
Abstract: As a new type of high performance liquid chromatographic technology, ion chromatography (IC) was initially mainly used to determine the content of anions and cations in samples. Ion chromatography has the advantages of simple operation, high sensitivity and good selectivity. In recent years, ion chromatography has been widely used in energy, environment, geology, food, medicine and other fields. The recent research progress of ion chromatography in traditional Chinese medicines such as metal ions, inorganic anions, organic acids, sugars were summarized in order to provide a reference for the study of ion chromatography in the analysis of chemical components of traditional Chinese medicine.
- traditional Chinese medicine /
- IC

HTML全文

表 1 IC在化学成分分析中的应用

Table 1. Application of IC in analysis of chemical components

来源	化学成分	色谱柱类型	参考文献
瓜蒌皮注射液	Cl^-、Na⁺、NH₄⁺	IonPac AS11-HC、IonPac CS12A	[7]
丹参	Li⁺、K⁺、Mg²⁺、Ca²⁺	IonPac CS12A	[8]
女贞子	Na⁺、K⁺、Mg²⁺、Ca²⁺	IonPac CS12A	[9]
萹蓄	K⁺、Mg²⁺、Ca²⁺	IonPac CS12A	[10]
40批中药制剂	SO₂/SO₄^2-	Thermo Scientific	[15]
甘草、制天南星和金银花等	SO₂/SO₄^2-	IonPac AS11-HC	[16]
西瓜霜	SO₂/SO₄^2-	IonPac AS11-HC	[17]
臭灵丹	SO₄^2-、PO₄^3-、Cl^-、NO₃^-	Ion Pac AS22	[20]
麻黄	SO₄^2-、Br^-、Cl^-、NO₂^-	Ion Pac AS22	[21]
漱口药	F^-、Cl^-、SO₄^2-、PO₃^4-、单氟磷酸钠、甘油、山梨酸钾及糖精钠	Ion Pac AS18	[22]
利咽灵片	F^-、HCOO^-、Cl^-、NO₂^-、Br^-、NO₃^-、SO₂^4-、C₂O₂^4-和PO₃^4-	Ion Pac	[23]
木瓜、女贞子、金银花、覆盆子、山茱萸	乳酸、乙酸、甲酸、苹果酸、酒石酸、草酸、富马酸	IonPac AS11-HC	[30]
金丝梅、萝芙木	NO₂^-、SO₄^2-、C₂O₂^4-、Cl^-	IonPac AS11	[31]
注射用丹参	乳酸、乙酸、甲酸、琥珀酸、草酸	IonPac AS11-HC	[32]
糊精	葡萄糖、麦芽糖、麦芽三糖、麦芽四糖、麦芽五糖	Dionex CarboPac PA200	[36]
乳制品	乳糖、半乳糖、葡萄糖、N-乙酰氨基葡萄糖	CarboPac PA1	[37]
果胶	葡萄糖、半乳糖、甘露糖、鼠李糖、阿拉伯糖、木糖	CarboPac PA1	[38]
玄参	水苏糖	METROSEP CARB 1	[39]
芦荟	岩藻糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖	Dionex CarboPac PA10	[41]
青钱柳	鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖	Carbo PAC^TM PA10	[42]
花黄精	鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、果糖	CarboPac PA1	[43]

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参考文献(43)

[1]	江静, 吴春敏. 离子色谱法在中药分析中的应用[J]. 海峡药学, 2013, 25(12): 24-26. https://www.cnki.com.cn/Article/CJFDTOTAL-HAIX201312009.htm JIANG Jing, WU Chun-min. The application of ion chromatography in the analysis of traditional Chinese medicine[J]. Strait Pharmaceutical Journal, 2013, 25(12): 24-26. https://www.cnki.com.cn/Article/CJFDTOTAL-HAIX201312009.htm
[2]	李永东. 基于离子色谱法的化学成分分析[J]. 化工管理, 2019(12): 189-190. doi: 10.3969/j.issn.1008-4800.2019.12.127 LI Yong-dong. Chemical composition analysis based on ion chromatography[J]. Chemical Enterprise Management, 2019(12): 189-190. doi: 10.3969/j.issn.1008-4800.2019.12.127
[3]	贾鹏禹, 孙蕊, 李良玉, 周行, 韩毅强, 冯乃杰. 生物样品中有机酸的离子排斥色谱测定方法进展[J]. 黑龙江八一农垦大学学报, 2020, 32(3): 123-128. doi: 10.3969/j.issn.1002-2090.2020.03.019 JIA Peng-yu, SUN Rui, LI Liang-yu, ZHOU Hang, HAN Yi-qiang, FENG Nai-jie. Research progress on determination of organic acids in biological samples by ion exclusion chromatography[J]. Journal of Heilongjiang Bayi Agricultural University, 2020, 32(3): 123-128. doi: 10.3969/j.issn.1002-2090.2020.03.019
[4]	崔静, 于小童. 反相离子对色谱法测定格列吡嗪片中5-甲基吡嗪-2-羧酸的含量[J]. 食品与药品, 2019, 21(5): 381-384. doi: 10.3969/j.issn.1672-979X.2019.05.011 CUI Jing, YU Xiao-tong. Determination of 5-methylpyrazine-2-carboxylic acid in glipizide tablets by reversed-phase ion-pair chromatography[J]. Food and Drug, 2019, 21(5): 381-384. doi: 10.3969/j.issn.1672-979X.2019.05.011
[5]	陈汀, 王玳珠, 李梅珍. 中药及汤剂中亚硫酸盐残留的研究现状[J]. 天津中医药大学学报, 2018, 37(4): 271-273. https://www.cnki.com.cn/Article/CJFDTOTAL-TZYY201804002.htm CHEN Ting, WANG Dai-zhu, LI Mei-zhen. Research status of sulfite residue in Chinese herbal medicine and decoction[J]. Journal of Tianjin University of Traditional Chinese Medicine, 2018, 37(4): 271-273. https://www.cnki.com.cn/Article/CJFDTOTAL-TZYY201804002.htm
[6]	王娜妮, 费莹, 王绪平, 寿旦, 朱岩. 离子色谱在中药分析中的应用研究[J]. 食品安全质量检测学报, 2015, 6(12): 4883-4888. https://www.cnki.com.cn/Article/CJFDTOTAL-SPAJ201512033.htm WANG Na-ni, FEI Ying, WANG Xu-ping, SHOU Dan, ZHU Yan. Application studies of the analysis of traditional Chinese medicine by ion chromatography[J]. Journal of Food Safety & Quality, 2015, 6(12): 4883-4888. https://www.cnki.com.cn/Article/CJFDTOTAL-SPAJ201512033.htm
[7]	程益清, 胡青, 毛秀红, 季申. 离子色谱法测定瓜蒌皮注射液中氯离子、钠离子和铵离子的含量[J]. 中国医药工业杂志, 2020, 51(3): 396-399. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHOU202003015.htm CHENG Yi-qing, HU Qing, MAO Xiu-hong, JI Shen. Determination of chloride, sodium and ammonium in Gualoupi injection by ion chrmatography[J]. Chinese Journal of Pharmaceuticals, 2020, 51(3): 396-399. https://www.cnki.com.cn/Article/CJFDTOTAL-ZHOU202003015.htm
[8]	翟武, 宣栋梁, 蔡嵘. 离子色谱法同时分析中药丹参中碱金属和碱土金属[J]. 理化检验-化学分册, 2002, 38(2): 66-67, 69. doi: 10.3321/j.issn:1001-4020.2002.02.005 ZHAI Wu, XUAN Dong-liang, CAI Rong. Simultaneous determination of alkali and alkaline earth metals in radix salviae miltiorrhizae by ion chromatography[J]. Physical Testing and Chemical Analysis Partb Chemical Analgsis, 2002, 38(2): 66-67, 69. doi: 10.3321/j.issn:1001-4020.2002.02.005
[9]	刘玉芬, 夏海涛, 葛洪玉. 离子色谱法测定中药女贞子浸煮液中的碱金属和碱土金属[J]. 广东微量元素科学, 2005, 12(8): 47-50. doi: 10.3969/j.issn.1006-446X.2005.08.013 LIU Yu-fen, XIA Hai-tao, GE Hong-yu. Determination of alkali and alkaline earth metal ions in soak and boil liquid of ligustrum lucidom by ion chromatography[J]. Trace Elements Science, 2005, 12(8): 47-50. doi: 10.3969/j.issn.1006-446X.2005.08.013
[10]	丁海萍, 赵玉英, 王琳琳. 离子色谱法对不同产地萹蓄中元素含量的比较研究[J]. 化学试剂, 2012, 34(6): 523-525. doi: 10.3969/j.issn.0258-3283.2012.06.011 DING Hai-ping, ZHAO Yu-ying, WANG Lin-lin. Comparative study of the elements content in polygonum aviculare L with different producing area by ICS method[J]. Chemical Reagents, 2012, 34(6): 523-525. doi: 10.3969/j.issn.0258-3283.2012.06.011
[11]	刘学民. 中药材经硫磺熏蒸后质量变化相关研究及对策分析[J]. 中西医结合心血管病电子杂志, 2019, 7(11): 29. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXJH201911019.htm LIU Xue-min. Correlation research and countermeasure analysis of quality change of Chinese medicinal materials after sulfur fumigation[J]. Cardiovascular Disease Electronic Journal of Integrated Traditional Chinese and Western Medicine, 2019, 7(11): 29. https://www.cnki.com.cn/Article/CJFDTOTAL-ZXJH201911019.htm
[12]	康传志, 杨婉珍, 莫歌, 周利, 蒋靖怡, 吕朝耕, 王升, 周涛, 杨野, 郭兰萍. 中药材二氧化硫限量标准及残留现状探讨[J]. 中国中药杂志, 2018, 43(2): 242-247. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201802007.htm KANG Chuan-zhi, YANG Wan-zhen, MO Ge, ZHOU Li, JIANG Jing-yi, LV Chao-geng, WANG Sheng, ZHOU Tao, YANG Ye, GUO Lan-ping. Sulfur dioxide limit standard and residues in Chinese medicinal materials[J]. China Journal of Chinese Materia Medica, 2018, 43(2): 242-247. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201802007.htm
[13]	中华人民共和国国家卫生和计划生育委员会. 食品安全国家标准食品添加剂使用标准: GB 2760—2014[S]. 北京: 中国标准出版社, 2015.
[14]	国家药典委员会. 中华人民共和国药典: 2020年版一部[S]. 北京: 中国医药科技出版社, 2020.
[15]	李梅花, 刘灿辉, 吕欣. 中药制剂中两种二氧化硫残留量测定方法的比较[J]. 临床合理用药杂志, 2019, 12(22): 117-118. https://www.cnki.com.cn/Article/CJFDTOTAL-PLHY201922067.htm LI Mei-hua, LIU Can-hui, LV Xin. Comparison of two determination methods of sulfur dioxide residue in traditional Chinese medicine preparations[J]. Chinese Journal of Clinical Rational Drug Use, 2019, 12(22): 117-118. https://www.cnki.com.cn/Article/CJFDTOTAL-PLHY201922067.htm
[16]	王欣美, 夏晶, 王柯, 季申. 碱性溶液提取-离子色谱法测定中药中二氧化硫残留量[J]. 中国中药杂志, 2011, 36(19): 2656-2659. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201119014.htm WANG Xinmei, XIA Jing, WNAG Ke, JI Shen. Determination of sulfur dioxide in traditional Chinese medicine by alkaline solution extraction and ion chromatography[J]. China Journal of Chinese Materia Medica, 2011, 36(19): 2656-2659. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201119014.htm
[17]	滕南雁, 梁飞燕. 离子色谱法测定中药西瓜霜硫酸盐的含量[J]. 药物分析杂志, 2011, 31(8): 1549-1551. https://www.cnki.com.cn/Article/CJFDTOTAL-YWFX201108032.htm TENG Nan-yan, LIANG Fei-yan. Ion chromatography quantitative determination of sulfates in mirabilitum praeparatum[J]. Chinese Journal of Pharmaceutical Analysis, 2011, 31(8): 1549-1551. https://www.cnki.com.cn/Article/CJFDTOTAL-YWFX201108032.htm
[18]	黄志芬, 李循媛, 伦麒景. 离子色谱法测定食品中二氧化硫残留量[J]. 海南师范大学学报(自然科学版), 2021, 34(2): 166-171. https://www.cnki.com.cn/Article/CJFDTOTAL-HNXZ202102009.htm HUANG Zhi-fen, LI Xun-yuan, LUN Qi-jing. Determination of sulfur dioxide in food by ion chromatography[J]. Journal of Hainan Normal University (Natural Science), 2021, 34(2): 166-171. https://www.cnki.com.cn/Article/CJFDTOTAL-HNXZ202102009.htm
[19]	王化同, 王侠, 于瑶. 浅谈离子色谱法测定当归中二氧化硫残留量[J]. 临床医药文献电子杂志, 2020, 7(48): 168-169. https://www.cnki.com.cn/Article/CJFDTOTAL-LCWX202048117.htm WANG Hua-tong, WANG Xia, YU Yao. Discussion on determination of residual sulfur dioxide in angelica by ion chromatography[J]. Electronic Journal of Clinical Medical Literature, 2020, 7(48): 168-169. https://www.cnki.com.cn/Article/CJFDTOTAL-LCWX202048117.htm
[20]	鲁广秋, 梁建平, 李松, 李亮星, 田先娇, 杨新周. 云南不同产区臭灵丹中4种无机阴离子的超声波辅助提取-离子色谱法含量测定研究[J]. 化学试剂, 2020, 42(10): 1201-1206. https://www.cnki.com.cn/Article/CJFDTOTAL-HXSJ202010014.htm LU Guang-qiu, LIANG Jian-ping, LI Song, LI Liang-xing, TIAN Xian-jiao, YANG Xin-zhou. Determination of four anions in laggera pterodonta (DC. ) Benth. from different areas of Yunnan by ion chromatography with ultrasonic-assisted extraction[J]. Chemical Reagents, 2020, 42(10): 1201-1206. https://www.cnki.com.cn/Article/CJFDTOTAL-HXSJ202010014.htm
[21]	符继红, 解成喜, 张丽静. 离子色谱法测定麻黄中的无机阴离子[J]. 色谱, 2004, 22(1): 72-73. https://www.cnki.com.cn/Article/CJFDTOTAL-SPZZ200401020.htm FU Ji-hong, XIE Cheng-xi, ZHANG Li-jing. Determination of inorganic anions in herba ephedrae by ion chromatography[J]. Chinese Journal of Chromatography, 2004, 22(1): 72-73. https://www.cnki.com.cn/Article/CJFDTOTAL-SPZZ200401020.htm
[22]	Zhang Y Z, Zhou Y C, Liu L, Zhu Y. Simultaneous determination of fluoride, chloride, sulfate, phosphate, monofluorophosphate, glycerophosphate, sorbate, and saccharin in gargles by ion chromatography[J]. Journal of Zhejiang University Science B, 2007, 8(7): 507-511. doi: 10.1631/jzus.2007.B0507
[23]	王宇, 黄勇. 离子色谱法同时检测利咽灵片中9种无机阴离子[J]. 中药材, 2017, 40(10): 2380-2382. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYCA201710033.htm WANG Yu, HUANG Yong. Simultaneous determination of nine inorganic anions in liyanling tablets by ion chromatography[J]. Journal of Chinese Medicinal Materials, 2017, 40(10): 2380-2382. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYCA201710033.htm
[24]	郑春松, 严培晶, 付长龙, 叶蕻芝, 吴广文, 李西海, 刘献祥. 从化合物-靶点作用网络的角度证实羌活抗炎镇痛的作用[J]. 风湿病与关节炎, 2017, 6(8): 10-14. https://www.cnki.com.cn/Article/CJFDTOTAL-FSBG201708003.htm ZHENG Chun-song, YAN Pei-jing, FU Chang-long, YE Hong-zhi, WU Guang-wen, LI Xi-hai, LIU Xian-xiang. Verifying the anti-inflammatory and analgesic effects of Qianghuo from the compound-target network perspective[J]. Rheumatism and Arthritis, 2017, 6(8): 10-14. https://www.cnki.com.cn/Article/CJFDTOTAL-FSBG201708003.htm
[25]	杨冰月, 李敏, 敬勇, 赖月月, 刘佳灵, 彭亮. 半夏及其炮制品化学成分及功效的差异研究[J]. 中草药, 2018, 49(18): 4349-4355. doi: 10.7501/j.issn.0253-2670.2018.18.020 YANG Bing-yue, LI Min, JING Yong, LAI Yue-yue, LIU Jia-ling, PENG Liang. Difference of chemical constituents and efficacy between crude and processed Pinelliae Rhizoma[J]. Chinese Traditional and Herbal Drugs, 2018, 49(18): 4349-4355. doi: 10.7501/j.issn.0253-2670.2018.18.020
[26]	雷智冬, 黄锁义. 草果提取方法、生物活性与临床应用研究新进展[J]. 中华中医药学刊, 2021, 39(1): 245-249. LEI Zhi-dong, HUANG Suo-yi. Research progress on extraction method, biological activity and clinical application of Caoguo(amomum tsao-ko)[J]. Chinese Archives of Traditional Chinese Medicine, 2021, 39(1): 245-249.
[27]	闫峻, 顾娟, 冯硕, 姬瑞芳, 全庆华, 刘永刚. 大青叶化学成分及抗氧化活性研究[J]. 质谱学报, 2019, 40(4): 381-390. https://www.cnki.com.cn/Article/CJFDTOTAL-ZPXB201904009.htm YAN Jun, GU Juan, FENG Shuo, JI Rui-fang, QUAN Qing-hua, LIU Yong-gang. Study of chemical constituents and antioxidant activity of isatidis folium[J]. Journal of Chinese Mass Spectrometry Society, 2019, 40(4): 381-390. https://www.cnki.com.cn/Article/CJFDTOTAL-ZPXB201904009.htm
[28]	雒军, 王引权, 吴国泰, 王艳, 王振恒, 荔淑楠, 彭桐. 当归根部中主要有效成分的动态积累和转化研究[J]. 中草药, 2021, 52(21): 6663-6668. LUO Jun, WANG Yin-quan, WU Guo-tai, WANG Yan, WANG Zhen-heng, LI Shu-nan, PENG Tong. Dynamic accumulation and transformation of ferulic acid and ligustilide in root of Angelica sinensis[J]. Chinese Traditional and Herbal Drugs, 2021, 52(21): 6663-6668.
[29]	汤喜兰, 刘建勋, 李磊. 中药有机酸类成分的药理作用及在心血管疾病的应用[J]. 中国实验方剂学杂志, 2012, 18(5): 243-246. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201205075.htm TANG Xi-lan, LIU Jian-xun, LI Lei. Pharmacological effects of organic acids in Chinese herbs and its application in cardiovascular diseases[J]. Chinese Journal of Experimental Traditional Medical Formulae, 2012, 18(5): 243-246. https://www.cnki.com.cn/Article/CJFDTOTAL-ZSFX201205075.htm
[30]	王娜妮, 张扬, 黄孝闻, 寿旦. 超声辅助离子色谱法分析5种中药饮片中有机酸含量[J]. 浙江中医杂志, 2015, 50(4): 310. https://www.cnki.com.cn/Article/CJFDTOTAL-ZJZZ201504060.htm WANG Na-ni, ZHANG Yang, HUANG Xiao-wen, SHOU Dan. Analysis of organic acid content in five kinds of traditional chinese medicine pieces by ultrasonic assisted ion chromatography[J]. Zhejiang Journal of Traditional Chinese Medicine, 2015, 50(4): 310. https://www.cnki.com.cn/Article/CJFDTOTAL-ZJZZ201504060.htm
[31]	李桂镇, 范伢, 穆凯艳, 覃安郡, 王根文, 庄艳, 包玉君, 王红斌. 固相萃取-离子色谱法测定中药萝芙木中常见有机酸和阴离子[J]. 分析试验室, 2015, 34(8): 913-917. https://www.cnki.com.cn/Article/CJFDTOTAL-FXSY201508010.htm LI Guizhen, FAN Ya, MU Kaiyan, QIN Anjun, WANG Genwen, ZHUANG Yan, BAO Yujun, WANG Hongbin. Determination of common organic acids and inorganic anions in rauvolfia plants by solid phase extraction-ion chromatography[J]. Chinese Journal of Analysis Laboratory, 2015, 34(8): 913-917. https://www.cnki.com.cn/Article/CJFDTOTAL-FXSY201508010.htm
[32]	刘静, 李静, 聂黎行, 王钢力, 王英新, 林瑞超. 离子色谱法同时测定注射用丹参(冻干)中有机酸和无机阴离子[J]. 药物分析杂志, 2012, 32(10): 1774-1777. https://www.cnki.com.cn/Article/CJFDTOTAL-YWFX201210016.htm LIU Jing, LI Jing, NIE Li-xing, WANG Gang-li, WANG Ying-xin, LIN Rui-chao. Simultaneous determination of organic acids and inorganic anions in Danshen for injection(lyophilized powder) by ion chromatography[J]. Chinese Journal of Pharmaceutical Analysis, 2012, 32(10): 1774-1777. https://www.cnki.com.cn/Article/CJFDTOTAL-YWFX201210016.htm
[33]	赵春霞, 余河水, 王春华, 李正. 中药中糖类定性及定量研究进展[J]. 中成药, 2019, 41(9): 2184-2192. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYA201909034.htm ZHAO Chun-xia, YU He-shui, WANG Chun-hua, LI Zheng. Advances in qualitative and quantitative research on carbohydrates in traditional Chinese medicine[J]. Chinese Traditional Patent Medicine, 2019, 41(9): 2184-2192. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYA201909034.htm
[34]	张锐波, 吕亚宁. 高效液相色谱示差法测定蜂蜜中的果糖、葡萄糖、蔗糖[J]. 食品安全导刊, 2019(15): 85. https://www.cnki.com.cn/Article/CJFDTOTAL-SPAQ201915075.htm ZHANG Rui-bo, LV Ya-ning. Determination of fructose, glucose and sucrose in honey by high performance liquid chromatography[J]. China Food Safety Magazine, 2019(15): 85. https://www.cnki.com.cn/Article/CJFDTOTAL-SPAQ201915075.htm
[35]	Liu X Y, Wang Y, Cong H L, Shen Y Q, Yu B. A review of the design of packing materials for ion chromatography[J]. Journal of Chromatography A, 2021, 1653: 462313.
[36]	李艺, 王淼. 离子色谱法测定糊精中的5种还原糖[J]. 广东药科大学学报, 2021, 37(1): 39-43. https://www.cnki.com.cn/Article/CJFDTOTAL-GDYX202101013.htm LI Yi, WANG Miao. Determination of five reducing sugars in dextrin by ion chromatography[J]. Journal of Guangdong Pharmaceutical University, 2021, 37(1): 39-43. https://www.cnki.com.cn/Article/CJFDTOTAL-GDYX202101013.htm
[37]	Cataldi T R I, Angelotti M, Bianco G. Determination of mono- and disaccharides in milk and milk products by high-performance anion-exchange chromatography with pulsed amperometric detection[J]. Analytica Chimica Acta, 2003, 485(1): 43-49.
[38]	Xu X B, Liu D B, Guo X M, Yu S J, Yu P. Improvement of sugar analysis sensitivity using anion-exchange chromatography-electrospray ionization mass spectrometry with sheath liquid interface[J]. Journal of Chromatography A, 2014, 1366: 65-72.
[39]	熊建飞, 周光明, 何强, 许丽, 张磊, 吴利敏. 离子色谱法测定玄参中的单糖和低聚寡糖[J]. 食品工业科技, 2012, 33(7): 340-343. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201207081.htm XIONG Jian-fei, ZHOU Guang-ming, HE Qiang, XU Li, ZHANG Lei, WU Li-min. Determination of monosaccharides and oligosaccharides in figwort root by ion chromatography[J]. Science and Technology of Food Industry, 2012, 33(7): 340-343. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201207081.htm
[40]	李娜, 余璇, 于巧红, 王萌. 中药多糖类成分稳定性研究进展[J]. 中国中药杂志, 2019, 44(22): 4793-4799. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201922003.htm LI Na, YU Xuan, YU Qiao-hong, WANG Meng. Research progress on stability of polysaccharides in traditional Chinese medicine[J]. China Journal of Chinese Materia Medica, 2019, 44(22): 4793-4799. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201922003.htm
[41]	乐胜锋, 王尉, 王雅萱, 赵新颖. 离子色谱-脉冲安培法测定芦荟多糖中7种单糖的含量[J]. 色谱, 2019, 37(3): 319-324. https://www.cnki.com.cn/Article/CJFDTOTAL-SPZZ201903013.htm YUE Sheng-feng, WANG Wei, WANG Ya-xuan, ZHAO Xin-ying. Determination of seven monosaccharides in aloe polysaccharide using ion chromatography with pulsed amperometric detector[J]. Chinese Journal of Chromatography, 2019, 37(3): 319-324. https://www.cnki.com.cn/Article/CJFDTOTAL-SPZZ201903013.htm
[42]	Xie J H, Shen M Y, Nie S P, Liu X, Zhang H, Xie M Y. Analysis of monosaccharide composition of cyclocarya paliurus polysaccharide with anion exchange chromatography[J]. Carbohydrate Polymers, 2013, 98(1): 976-981.
[43]	何连军, 干雅平, 吕伟德, 饶君凤, 杨菊妹, 余家胜. 高效阴离子交换色谱-脉冲安培检测法测定多花黄精多糖的单糖组成[J]. 中草药, 2017, 48(8): 1671-1676. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201708029.htm HE Lian-jun, GAN Ya-ping, LV Wei-de, RAO Jun-feng, YANG Ju-mei, YU Jia-sheng. Monosaccharide composition analysis on polysaccharides in polygonatum cyrtonema by high performance anion-exchange chromatography with pulsed amperometric detection[J]. Chinese Traditional and Herbal Drugs, 2017, 48(8): 1671-1676. https://www.cnki.com.cn/Article/CJFDTOTAL-ZCYO201708029.htm

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来源	化学成分	色谱柱类型	参考文献
瓜蒌皮注射液	Cl^-、Na⁺、NH₄⁺	IonPac AS11-HC、IonPac CS12A	[7]
丹参	Li⁺、K⁺、Mg²⁺、Ca²⁺	IonPac CS12A	[8]
女贞子	Na⁺、K⁺、Mg²⁺、Ca²⁺	IonPac CS12A	[9]
萹蓄	K⁺、Mg²⁺、Ca²⁺	IonPac CS12A	[10]
40批中药制剂	SO₂/SO₄^2-	Thermo Scientific	[15]
甘草、制天南星和金银花等	SO₂/SO₄^2-	IonPac AS11-HC	[16]
西瓜霜	SO₂/SO₄^2-	IonPac AS11-HC	[17]
臭灵丹	SO₄^2-、PO₄^3-、Cl^-、NO₃^-	Ion Pac AS22	[20]
麻黄	SO₄^2-、Br^-、Cl^-、NO₂^-	Ion Pac AS22	[21]
漱口药	F^-、Cl^-、SO₄^2-、PO₃^4-、单氟磷酸钠、甘油、山梨酸钾及糖精钠	Ion Pac AS18	[22]
利咽灵片	F^-、HCOO^-、Cl^-、NO₂^-、Br^-、NO₃^-、SO₂^4-、C₂O₂^4-和PO₃^4-	Ion Pac	[23]
木瓜、女贞子、金银花、覆盆子、山茱萸	乳酸、乙酸、甲酸、苹果酸、酒石酸、草酸、富马酸	IonPac AS11-HC	[30]
金丝梅、萝芙木	NO₂^-、SO₄^2-、C₂O₂^4-、Cl^-	IonPac AS11	[31]
注射用丹参	乳酸、乙酸、甲酸、琥珀酸、草酸	IonPac AS11-HC	[32]
糊精	葡萄糖、麦芽糖、麦芽三糖、麦芽四糖、麦芽五糖	Dionex CarboPac PA200	[36]
乳制品	乳糖、半乳糖、葡萄糖、N-乙酰氨基葡萄糖	CarboPac PA1	[37]
果胶	葡萄糖、半乳糖、甘露糖、鼠李糖、阿拉伯糖、木糖	CarboPac PA1	[38]
玄参	水苏糖	METROSEP CARB 1	[39]
芦荟	岩藻糖、鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖	Dionex CarboPac PA10	[41]
青钱柳	鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、木糖	Carbo PAC^TM PA10	[42]
花黄精	鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖、果糖	CarboPac PA1	[43]

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离子色谱法在中药化学成分分析中的应用进展

doi: 10.16495/j.1006-3757.2022.02.012

作者简介:
颉东妹(1996-), 女, 硕士研究生, 研究方向: 中药学, E-mail: 416174332@qq.com

通讯作者:
郭玫，女，教授，研究方向：中药药效物质基础与质量标准研究，E-mail: guomei@gszy.edu.com

邸多隆，男，研究员，《分析测试技术与仪器》副主编，研究方向：分析化学，E-mail: didl@licp.cas.cn

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Application Progress of Ion Chromatography in Analysis of Chemical Components of Traditional Chinese Medicine

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离子色谱法在中药化学成分分析中的应用进展

doi: 10.16495/j.1006-3757.2022.02.012

作者简介: 颉东妹(1996-), 女, 硕士研究生, 研究方向: 中药学, E-mail: 416174332@qq.com

通讯作者: 郭玫，女，教授，研究方向：中药药效物质基础与质量标准研究，E-mail: guomei@gszy.edu.com 邸多隆，男，研究员，《分析测试技术与仪器》副主编，研究方向：分析化学，E-mail: didl@licp.cas.cn

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Application Progress of Ion Chromatography in Analysis of Chemical Components of Traditional Chinese Medicine

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期刊在线

读者中心

友情链接

作者简介:
颉东妹(1996-), 女, 硕士研究生, 研究方向: 中药学, E-mail: 416174332@qq.com

通讯作者:
郭玫，女，教授，研究方向：中药药效物质基础与质量标准研究，E-mail: guomei@gszy.edu.com

邸多隆，男，研究员，《分析测试技术与仪器》副主编，研究方向：分析化学，E-mail: didl@licp.cas.cn