行波离子迁移谱技术及应用研究进展

潘慢慢; 李杭; 徐一仟; 杨其穆; 蒋丹丹; 王卫国; 陈创; 李海洋

doi:10.16495/j.1006-3757.2023.03.001

行波离子迁移谱技术及应用研究进展

doi: 10.16495/j.1006-3757.2023.03.001

潘慢慢^{1, 2,},
李杭²,
徐一仟^{1, 2},
杨其穆^{1, 2},
蒋丹丹²,
王卫国²,
陈创^{2, 3, ,},
李海洋^2, ,

1.
中国科学院大学，北京　100049
2.
中国科学院大连化学物理研究所，辽宁大连　116023
3.
国民核生化灾害防护国家重点实验室，北京　102205

基金项目: 国家自然科学基金项目（Nos. 22027804, 21974141），国民核生化灾害防护国家重点实验室科研基金项目（SKLNBC2021-16），大连化物所创新研究基金项目（DICP I202141）

详细信息

作者简介:
潘慢慢（1998−），女，博士研究生，主要从事质谱分析工作，E-mail：panmanman@dicp.ac.cn

通讯作者:
陈创（1984−），男，博士，《分析测试技术与仪器》青年编委，主要从事质谱分析工作，E-mail：chenchuang@dicp.ac.cn

李海洋（1964−），男，博士，《分析测试技术与仪器》编委，主要从事质谱分析工作，E-mail：hli@dicp.ac.cn

中图分类号: O657. 63
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- 文章访问数: 74
- HTML全文浏览量: 21
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- 被引次数: 0
出版历程
- 收稿日期: 2023-05-24
- 录用日期: 2023-07-13
- 修回日期: 2023-07-13
- 刊出日期: 2023-09-25

Advancement of Traveling Wave Ion Mobility Spectrometry and Its Application

PAN Manman^{1, 2
,},
LI Hang²,
XU Yiqian^{1, 2},
YANG Qimu^{1, 2},
JIANG Dandan²,
WANG Weiguo²,
CHEN Chuang^{2, 3
, ,},
LI Haiyang^{2
, ,}

1.
University of Chinese Academy of Sciences, Beijing 100049, China
2.
Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning China
3.
State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China

Funds: Natural Science Foundation of China (Nos. 22027804, 21974141), State Key Laboratory of NBC Protection for Civilian (SKLNBC2021-16), Dalian Institute of Chemical Physics (DICP I202141)

摘要

摘要: 离子迁移谱（ion mobility spectrometry，IMS）是利用离子迁移率K（离子碰撞截面）差异来实现不同离子的分离与测定，具有分析速度快、检测灵敏度高的优点，其与质谱联用在蛋白质组学、代谢组学、医药等领域已获得了广泛的应用. 随着分析对象复杂性的增加，对IMS的分辨能力也提出了更高要求. 行波离子迁移谱（travelling wave ion mobility spectrometry，TWIMS）采用时域连续的行波电场实现离子传输与分离，其分析通道的长度不受行波电压幅值的限制，理论上可以无限延长离子分析通道来提高分辨能力. 目前，TWIMS的分辨率最高可达1 860，对于分析存在多种同分异构体的复杂样品别具优势. 对TWIMS的原理及分辨能力的影响因素进行了介绍，进一步探讨了不同结构TWIMS仪器的特点、性能和应用，对TWIMS未来发展方向进行了展望.
- 离子碰撞截面 /
- 行波离子迁移谱 /
- 循环式离子迁移谱 /
- 无损离子操纵结构 /
- 离子淌度质谱
Abstract: Ion mobility spectrometry (IMS) utilizes the difference in ion mobility K (collision cross section) to realize the separation and determination of different ions, which has the advantages of fast analysis speed and high sensitivity. And it coupling with mass spectrometry (IM-MS) was widely used in the fields of proteomics, metabolomics, medicine, etc. With the increasing complexity of the analyzed objects, higher demands are put on the resolution of the IMS. Traveling wave ion mobility spectrometry (TWIMS) uses a time-domain continuous traveling wave electric field to realize ion transport and separation. The analytical path length of the TWIMS is not limited by the amplitude of the travelling wave voltage, theoretically the path can be extended indefinitely to improve the resolution. Currently, the resolution of TWIMS can reach up to 1 860, which is advantageous for the analysis of complex samples with the multiple isomers. The principle of TWIMS and the influencing factors of resolution were introduced, the characteristics, performance and applications of TWIMS instruments with different structures were further discussed, and finally the future development directions of TWIMS were prospected.
- collision cross section /
- travelling wave ion mobility spectrometry /
- cyclic ion mobility spectrometry /
- structure for lossless ion manipulation /
- ion mobility-mass spectrometry

HTML全文

图 1 行波场中离子（a）（c）翻滚事件和（b）“冲浪”行为的SIMION轨迹模拟，（d）行波场的产生^[14]

Figure 1. SIMION simulation showing ions (a) (c) roll over wave and (b) surf wave, (d) generation of travelling wave^[14]

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图 2 （a）第一代行波离子迁移管^[14]，（b）Synapt HDMS示意图^[18]

Figure 2. (a) First generation TWIM separator^[14], (b) schematic diagram of Synapt HDMS system^[18]

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图 3 Synapt HDMS和Synapt G2 HDMS的（a）IM腔室，行波电压（b）施加方式和（c）重复模式对比图^[19]

Figure 3. Comparison of (a) IM cells, (b) applied voltage and (c) repeat pattern of travelling wave between Synapt HDMS and Synapt G2 HDMS^[19]

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图 4 cIM的结构示意图^[35]

（a）cIM平台概览，（b）cIM设备，（c）包含阵列电极结构的离子注入/喷射区域，（d）cIM电极结构，（e）离子注入/喷射模式下行波方向为x或-x，（f）分离模式下行波方向为y

Figure 4. Schematic diagram of structure of cIM ^[35]

(a) overview of cIM plateform, (b) cIM device, (c) ion entry/exit region, consisting of array electrodes, (d) structure of cIM electrodes, (e) ion injection/ejection mode, array TWs applied in x (or -x) direction, (f) separation mode, array TWs applied in y-direction

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图 5 激活的+7 CytC离子的（a）到达时间分布，（b）16~17 ms、（c）19~20 ms、（d）23~24 ms和（e）26~27 ms切片的CIU指纹，α、β、γ、δ、ε和ζ表示离子种群^[37]

Figure 5. (a) Arrival time distribution of activated +7 CytC ion, and CIU fingerprints for slices (b) 16~17 ms, (c) 19~20 ms, (d) 23~24 ms, (e) 26~27 ms, populations labeled as α, β, γ, δ, ε and ζ^[37]

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图 6 TW-SLIM的电极结构^[47]

Figure 6. Structure of electrodes in TW-SLIM^[47]

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图 7 （a）13 m长的蛇形TW-SLIM^[49]，（b）循环蛇形路径TW-SLIM和动态离子开关^[9]

Figure 7. (a) 13 m serpentine path length TW-SLIM^[49], (b) serpentine ultralong path with extended routing TW-SLIM and dynamic ion switch^[9]

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图 8 （a）1次和（b）9次通过后获得的乳-N-六糖和乳-N-新六糖的迁移率分离结果^[9]

Figure 8. IM-MS separation of sugar isomers lacto-N-hexaose and lacto-N-neohexaose obtained at (a) 1 pass and (b) 9 passes^[9]

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图 9 （a）SLIM-QQQ平台示意图，每条离子路径中包含的（b）SOBA、（c）预过滤区域、（d）过滤门和（e）出口三通的图示^[60]

Figure 9. (a) Schematic diagram of SLIM-QQQ platform, illustration of different subsections including (b) SOBA, (c) pre-filter, (d) mobility filter gate and (e) exit tee in each ion path^[60]

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图 10 miniSLIM结构图^[62]

Figure 10. Structure diagram of miniSLIM^[62]

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表 1 不同IMS技术对比

Table 1. Comparison of different IMS

IMS技术	工作气压^[10]	分离场	CCS测量	最高分辨率/(Ω/ΔΩ)	联用技术
迁移时间离子迁移谱（DTIMS）	266 Pa~大气压强	直流电场	直接测量	250^[11]	IMS-MS, GC (gas chromatography)-IMS等
非对称场离子迁移谱（FAIMS/DMS）	大气压强	非对称射频电场	无法测量	Null	GC-DMS, DMS-MS等
行波场离子迁移谱（TWIMS）	~533 Pa	方波直流电场	需经校准	1 860^[9] 550^[12]	TWIMS-MS
阱离子迁移谱（TIMS）	~400 Pa	气流场结合直流电场	需经校准	400^[13]	TIMS-MS

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