木质纤维素材料前处理的方法

王小曼; 王闻

doi:10.16495/j.1006-3757.2023.03.010

木质纤维素材料前处理的方法

doi: 10.16495/j.1006-3757.2023.03.010

王小曼,
王闻

中国科学院广州能源研究所，广东广州　510630

基金项目: 国家重点研发项目（No.2019YFC1905301-1）

详细信息

作者简介:
王小曼（1991−），女，工程师，主要从事电镜测试与样品前处理工作，E-mail：wangxm@ms.giec.ac.cn

中图分类号: O657; TN16
计量
- 文章访问数: 23
- HTML全文浏览量: 16
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2023-04-25
- 录用日期: 2023-06-21
- 修回日期: 2023-06-21
- 刊出日期: 2023-09-25

Pretreatment Methods of Lignocellulosic Materials

Guangzhou Institute of Energy Conversion Academy of Sciences, Guangzhou 510630, China

Funds: National Key Research and Development Project (No.2019YFC1905301-1)

摘要

摘要: 木质纤维素类材料在地球上大量存在，来源广泛. 对木质纤维素废料进行处理，可以废物回收，避免环境问题. 由于木质纤维素材料细胞壁较厚，细胞壁中含结晶结构，进行透射电子显微镜观察前需经过前处理. 对木质纤维素材料的样品前处理方法进行了详细说明，包括取材、固定、洗涤、脱水、包埋和渗透、切片及染色，可为其制备提供参考.
- 木质纤维素 /
- 前处理 /
- 透射电子显微镜 /
- 切片
Abstract: The lignocellulosic materials exist in large quantities on the earth and come from a wide range of sources. The treatment of lignocellulosic waste allows for the recycling of waste, and avoids environmental problems. Due to the thick cell walls of lignocellulosic materials and the cell walls contains crystalline structures, pretreatment is required before transmission electron microscopic observation. The sample pretreatment methods of lignocellulosic materials were described in detail, including sampling, fixation, washing, dehydration, resin embedding and penetration, slicing and dyeing, so as to provide a reference for the preparation of the kind of materials.
- lignocellulose /
- pretreatment /
- transmission electron microscopy /
- slicing

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图 1 修块示意图

Figure 1. Schematic diagram of trimming

下载: 全尺寸图片幻灯片

图 2 对刀示意图

Figure 2. Schematic diagram of tool setting

下载: 全尺寸图片幻灯片

图 3 （左图）未经处理的和（右图）NaOH处理的木质纤维素材料的TEM图像

（a）小麦秸秆，（b）玉米秸秆，（c）甘蔗渣，（d）柏树，（e）紫檀（CC：细胞角，CML：复合中间层，PCW：初级细胞壁，SCW：次级细胞壁）^[4]

Figure 3. TEM images of (left) untreated and (right) NaOH-treated lignocellulosic materials (a) wheat straw, (b) corn straw, (c) sugarcane bagasse, (d) cypress, (e) pterocarpus soyauxii (CC: cell corner, CML: compound middle lamella, PCW: primary cell wall, SCW: secondary cell wall) ^[4]

下载: 全尺寸图片幻灯片

参考文献(9)

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