Abstract: By optimizing the pretreatment conditions, a method for the determination of 16 polycyclic aromatic hydrocarbons (PAHs) in Lilium davidii var. unicolor by gas chromatography-mass spectrometry (GC-MS) has been established. The samples were infiltrated with deionized water, extracted with acetonitrile, and dehydrated with mixed powder of MgSO₄ and NaCl. After extraction, the samples were concentrated to 2 mL. The samples were purified by a Carb/NH₂ solid phase extraction column, dissolved in n-hexane, separated by a DB-5MS capillary column (Agilent, 30 m×250 μm, 0.25 μm), analyzed by GC-MS, and quantified by external standard method. The correlation coefficients of 16 PAHs were all greater than 0.99, the limits of detection were 0.2~0.8 μg/kg, the limits of quantification were 0.9~3.1 μg/kg, and the relative standard deviations were 1.75%~5.14%. The method is reliable, sensitive and accurate, and can be used for daily monitoring and detection of PAHs in the Lilium davidii var. unicolor.