7-甲基鸟苷在玻碳电极上的伏安行为研究

The Study of the Voltammetric Behaviour of 7-methylguanosineon Glassy Carbon Electrode

摘要: 研究了修饰核苷7-甲基鸟苷（7-Methylguanosine）的电化学行为及测定方法.在pH 1.98的B-R缓冲液中，用循环伏安法（CV），线性扫描伏安法（LSV）、微分脉冲伏安法（DPV）等现代电化学技术研究7-甲基鸟苷在玻碳电极（GCE）上的伏安行为.实验表明，7-甲基鸟苷在＋1.036 V（vs. SCE）电位处产生一个阳极氧化峰，峰电流与7-甲基鸟苷的浓度在5.0×10^-5～2.0×10^-6 mol/L范围内呈良好的线性关系，最低检测限（D=2σ/K）为4.1×10^-7 mol/L.并用恒电位库仑电解法等方法对其氧化机理进行了较为详细的探讨，得到了可能的电极反应机理：7-甲基鸟苷在玻碳电极上的电极反应是属于失1质子和2电子的不可逆的氧化反应.

Abstract: The electrochemical response of 7-methylguanosine in pH 1.98 B-R buffer was investigated by cyclic voltammetry (CV), linear sweep voltammetry (LSV) and differential pulse voltammetry (DPV) method. The differential pules voltammetry exhibited an oxidation current peak of 7-methylguanosine at the potential of +1.036 V (vs. SCE). The peak current is linearly increased with the concentration of 7-methylguanosine in the range of 5.0×10^-5~2.0×10^-6 mol/L. The detection limit is 4.1×10^-7 mol/L. The possible mechanism for the irreversible reaction was assumed to be the oxidation lossing of one proton and two electrons.