Abstract: Two enatiomers of indole ring derivatives have been chiral separated on amylase-tris (3,5-dimethylphenylcarbamate) chiral stationary phase (CSP), using hexane as the mobile phase with various alcohols as modifiers. The influence of the mobile phase composition on the enantioselectivity was discussed. By comparing the results from amylase-tris (3,5-dimethylphenylcarbamate) chiral stationary phase and cellulose tris (3, 5-dimethylphenylcarbamate) chiral stationary phase, the separation abilities are different on the two stationaries.