Abstract: The determination of dihydroactinidiolide was carried out by GC/MS 5973 Agilent with HP-5MS capillary column and the selective ion measurement(SIM) method. Under the optimum conditions, the detection limit was found to be 0.1 ng,and linearity was held in the range of 0.5~100 ng of dihydroactinidiolide in 1 μL of the sample solution. Recovery test was made by the standard addition method and the value of 99.8% was obtained,and the RSD′s (n=6) obtained were 1.04% .