Abstract: A method for the determination of 16 phthalates in the health care wine by gas chromatography-triple quadrupole mass spectrometry(GC-MS/MS) was developed. Pretreatment methods of phthalate esters in health care wine were studied and optimized. An extraction method to remove ethanol on a boiling water bath was established, using n-hexane as the extractant. Quantitative analysis was performed on the time selective reaction scanning mode (T-SRM). The chromatographic column used was a TG-5MS quartz capillary column (30 m×0.25 mm×0.25 μm), the programmed temperature (initial column temperature was 60℃, kept for 1 min, and raised to 220℃ at 20℃/min, kept for 1 min, then kept at 5℃/min to 280℃, kept for 4 min), without shunting. The results showed that the 16 phthalates had an effective internal correlation (R² > 0.995). The average recovery was 84.0%~112.8%, and the RSD was less than 7% (n=6). 6 PAEs in health care wine were determined by this method. The results showed that the method is sensitive, simple and rapid, accurate and reliable. The GC-MS/MS method can satisfy the qualitative analysis and quantitative determination of the phthalates in health care wine.