Abstract: To establish a gas chromatography-mass spectrometry(GC/MS) method with supported liquid extraction(SLE) for the determination of common sedative hypnotic drugs in blood. Five sedative hypnotic drugs, amitriptyline, estazolamum, midazolam, promethazine and chlorpromazine, were determined by GC/MS after being processed with SLE, and experimental conditions were optimized. After adding five sedative hypnotic drugs in the blank blood, they were separated and determined by SLE-GC/MS. Optimal experimental conditions were:pH 10 and 5.0 mL of ethyl acetate as the extraction solvent. The results showed a good linearity among the concentrations of 0.5~20 μg/mL of the five drugs and the peak areas with correlation coefficients of 0.998 1~0.999 5, the limits of quantification(LOQ) were approximately 0.5 μg/mL, the average recoveries were 70.1%~90.8%, and the coefficient of variation was less than 5. 3%. The method is simple, rapid, with a high recovery, and it can be used in the determination of common sedative hypnotic drugs in blood.