Abstract: A method for the determination of lidocaine hydrochloride in lincomycin hydrochloride and lidocaine hydrochloride gel by high performance liquid chromatography(HPLC)is set up. Agilent 5 Tc-C18(2)(250 mm×4.6 mm, 5 μm)column was used. The mobile phase consisted of 0.05 mol/L Borax (adjusted to pH 6.0 with phosphoric acid)-methanol-acetonitrile(68:19:13). The UV detection wavelength was at 214 nm, with a flow rate of 1 mL/min and the column temperature was 30℃. The linearity was good for lidocaine within the range of 40.1~240.6 μg/mL(r=0.999 4), the sample recovery rate was 97.6%. The established method is operative and simple, and can be used for the quality control of lidocaine hydrochloride in preparations.