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欧阳丹, 吴杰, 谢更奇, 郑梓扬, 施涵强, 陈锋, 陈玉谊, 林子俺. 基于MnO2/石墨烯纳米材料的表面辅助激光解吸电离质谱在内毒素快速鉴定中的应用[J]. 分析测试技术与仪器, 2019, 25(2): 72-77. DOI: 10.16495/j.1006-3757.2019.02.002
引用本文: 欧阳丹, 吴杰, 谢更奇, 郑梓扬, 施涵强, 陈锋, 陈玉谊, 林子俺. 基于MnO2/石墨烯纳米材料的表面辅助激光解吸电离质谱在内毒素快速鉴定中的应用[J]. 分析测试技术与仪器, 2019, 25(2): 72-77. DOI: 10.16495/j.1006-3757.2019.02.002
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OUYANG Dan, WU Jie, XIE Geng-qi, ZHENG Zi-yang, SHI Han-qiang, CHEN Feng, CHEN Yu-yi, LIN Zi-an. MnO2/Graphene Nanomaterials-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Rapid Identification of Endotoxin[J]. Analysis and Testing Technology and Instruments, 2019, 25(2): 72-77. DOI: 10.16495/j.1006-3757.2019.02.002
Citation: OUYANG Dan, WU Jie, XIE Geng-qi, ZHENG Zi-yang, SHI Han-qiang, CHEN Feng, CHEN Yu-yi, LIN Zi-an. MnO2/Graphene Nanomaterials-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Rapid Identification of Endotoxin[J]. Analysis and Testing Technology and Instruments, 2019, 25(2): 72-77. DOI: 10.16495/j.1006-3757.2019.02.002
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基于MnO2/石墨烯纳米材料的表面辅助激光解吸电离质谱在内毒素快速鉴定中的应用

MnO2/Graphene Nanomaterials-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Rapid Identification of Endotoxin

    摘要
  • 摘要: 利用大肠埃希菌（E.coli O111：B4）中提取精制的内毒素（Control standard endotoxin，CSE）为研究对象，以MnO2/石墨烯（MnO2/G）纳米复合材料为基质，建立了一种基于MnO2/G纳米材料的表面辅助激光解吸电离质谱（Surface-assisted laser desorption ionization mass spectrometry，SALDI-MS）的内毒素检测新方法.利用SALDI-MS方法可实现对不同注射液和饮用水中内毒素的快速鉴定与定量分析.与传统的鲎试剂检测方法相比，基于MnO2/G纳米复合材料的SALDI-MS方法具有操作简便、灵敏度高、分辨率高、检测速度快、高通量和耐盐性好等优点，有望应用于更多食品和药品中细菌内毒素的高通量快速筛查.

     

    Abstract: A new method for the surface-assisted laser desorption ionization mass spectrometry (SALDI-MS) was developed using MnO2/graphene (MnO2/G) nanocomposites as the matrix. The control standard endotoxin (CSE) extracted from Escherichia coli (E.coli O111:B4) was used as the target and the SALDI-MS method for the analysis of CSE was set up, which could be successfully applied to the rapid identification and quantitative analysis of endotoxin in different injections and drinking water. Compared with the traditional method of tachypleus amebocyte lysate (TAL), the MnO2/G nanocomposites-based SALDI-MS method is of shows high sensitivity, high mass resolution, rapid analysis, high throughput, and good salt tolerance. The successful applications suggest the potential of the SALDI-MS for the rapid high-throughput screening of CSE in foods and drugs.

     

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