Abstract: The multimode small animal imaging system is widely used in the detection and research of living animals in scientific ressearch laboratories of colleges and universities, which provides an intuitive in vivo detection method for the occurrence and development of cancer, gene therapy, immunology and molecular biology. Combined with the application experience, the application and development of multimode small animal imaging system in vivo for innovative practice of undergraduates is discussed. The purpose is to improve the efficiency of multimode small animal imaging system, expand the experimental resources of multi-mode small animal imaging system.