Abstract: According to the principle of equal absorption dual-wavelength elimination colorimetric method, two starch contents were determined by the developed portable speed measuring apparatus for amylose and amylopectin in food. The validation test results indicated that the results of linear response ranges (R²>0.999), precision (RSD% < 0.7%), stability (RSD% < 1.5%), repeatability (RSD% < 2.0%), reproducibility (RSD% < 2.5% in the same laboratory and RSD% < 4.2% in different laboratories), recovery (average recovery was more than 90%, RSD% < 2.5%), system applicability (RSD% < 2.0% for each apparatus and RSD% < 3.5% between different apparatuses) were good, respectively. The detection process is simple, fast and accurate, the detection wavelength and reference wavelength of the two starches which were integrated in the single-chip microcomputer system of the instrument device were used directly. So these parameters were no need to be set again during the experiment and only two times of the absorbance differences were need to be determined. The operation time was saved and the sample detection efficiency was improved. The development of this portable speed measuring apparatus provides a powerful technical guarantee for the rapid and accurate detection of two different starches as in large batches of samples and it is an important technological innovation in the functional development of the function of speed measuring apparatus for starch.