Abstract: As a new typical sample pretreatment approach, magnetic solid-phase extraction (MSPE) has been widely applied in the separation and purification of complex samples, which has many advantages, including costing less extraction time and organic solvents, having higher absorption ability and simple and fast operation. The development of MSPE and materials were reviewed, and the application in toxicological analysis was also discussed in detail, aiming at provide reference for the trace evidence analysis in forensic science area and cracking down the drug crime.