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刘时婉, 张世敏. 草鱼出血病病毒提纯中离心方法和离心参数的选择[J]. 分析测试技术与仪器, 1994, (2): 33-36.
引用本文: 刘时婉, 张世敏. 草鱼出血病病毒提纯中离心方法和离心参数的选择[J]. 分析测试技术与仪器, 1994, (2): 33-36.
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Liu Shiwan, Zhang Shimin. The Choice of Centrifugation Method and Parameters in the Isolation of Grass Carp Haemorrhage Virus[J]. Analysis and Testing Technology and Instruments, 1994, (2): 33-36.
Citation: Liu Shiwan, Zhang Shimin. The Choice of Centrifugation Method and Parameters in the Isolation of Grass Carp Haemorrhage Virus[J]. Analysis and Testing Technology and Instruments, 1994, (2): 33-36.
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草鱼出血病病毒提纯中离心方法和离心参数的选择

The Choice of Centrifugation Method and Parameters in the Isolation of Grass Carp Haemorrhage Virus

    摘要
  • 摘要: 为了有效地从鱼病组织中初提纯草鱼出血病病毒,我们探讨了各种离心方法和离心参数,结果表明最佳离心方法为角度离心(日立20PR-52D离心机,RPR20-2转头),最佳离心参数为：转头温度是8℃,离心速度是16000转/分,离心时间是4.5小时。该离心方法和离心参数已成为我们实验室最常规使用的提纯该病毒的方法。

     

    Abstract: For more effichat isolation of Grass Carp Haemorrhage Virus(GCHV) from infected fish tissue,.differenth methods and different parameters of centrifugation were extlored. It was found that the most suitable centrifugation method angular centrifugation and the most suitable Parameters for isolating typical viral particles were: revolution speed 16000rpm; duration.4.5h, rotor temperatrre, 8℃ (Hitachi 20 PR-52D Centrifuge, RPR 20-2 Rotor). Thiscentrifugation method is now used routinely in our laboratory for the isolation of GCHV.

     

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