Abstract: The preparation of macroporous silica matrices of different pore diameter, the bonding of polymer to 7μm different pore diameter silica for the preparation of a novel packing has been studied. The chromatographic characteristics of these packing were examined. The results showed that under gradient elution, Proteins (pepsin,ribonuclease, lysozyme, chymotrypsinogen, ovalbumin)were separated within 15min successfully. Similar structure Amino-acid were separated on base line less than 3min.It is suggested that these packings separate biopolymer quickly with high resolution, and they have a wide-range applied prospective.