Abstract: Flavonoids are widely distributed in the plant kingdom and over 4 000 different flavonoids have been identified. Most of these compounds are strong anti-oxidants that can be found in vegetables, fruits, wine, tea, and so on, so flavonoids play a most important role in human health. In order to separate and determine the flavonoids in many plants more convenient, the review with 43 references was presented to introduce the analytical methods of quantity and quality of natural product flavonoids, including flavone, flavonol and flavanone and other type with HPLC. Meantime fingerprints and pharmacokinetic were also brief introduced.