红枣中果糖、葡萄糖与蔗糖的高效液相色谱示差检测分析

Simultaneous Determination of Fructose, Glucose and Sucrose in Red Jujube by High Performance Liquid Chromatography with Differential Detector

摘要: 建立了同时分析鲜红枣中果糖、葡萄糖和蔗糖的高效液相色谱方法. 利用鲜红枣样品进行添加回收实验，获得方法对果糖、葡萄糖和蔗糖测定的平均回收率分别为93.5%、91.8%和89.8%，变异系数3.27%、2.34%、5.86%. 结果表明：采用高效液相色谱示差分析方法定量检测鲜红枣中果糖、葡萄糖和蔗糖快速、简便. 同时得出方法的检出限为：果糖5.73×10^-3mg/mL，葡萄糖5.22×10^-3 mg/mL，蔗糖3.59×10

Abstract: A method for the simultaneous determination of fructose,glucose and sucrose in red jujube was established by using a high performance liquid chromatograph with a differential detector. The average recoveries of the method for fructose, glucose and sucrose determination in spiked red jujube samples were 93.5%,91.8% and 89.8% with the variation coefficient ranged from 3.27%,2.34%,5.86% respectively.The results showed that the established method is simple and fast in the determination of fructose, and glucose, and sucrose in red jujube. The method detection limits were: fructose, 5.73×10^-3 mg/mL,glucose, 5.22×10^-3 mg/mL, sucrose, 3.59×10^-3mg/mL, the measured results meet the food testing requirements.