Abstract: Three cellulose-based chiral columnscellulose tris(4-methylphenylcarbamate) (ATEO-OG), tris(3, 5-dimethylphenylcarbamate) (ATEO-OD), and tris(4-methyl benzoate) (ATEO-OJ) were prepared in our laboratory. The comparasion and chiral separation of sixteen chiral compounds on the obtained cellulose-based chiral columns have been described. The effect of chromatographic conditions on chiral separation was investigated. The results showed that 16 chiral compounds were separated chirally with different degree on the three chiral stationary phases, and the chiral recognition ability of ATEO-OD was higher than the other two, baseline or partial enantioseparation of 16 compounds was obtained. ATEO-OG and ATEO-OJ has the same chiral recognition ability.