MnO₂/Graphene Nanomaterials-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Rapid Identification of Endotoxin

A new method for the surface-assisted laser desorption ionization mass spectrometry (SALDI-MS) was developed using MnO₂/graphene (MnO₂/G) nanocomposites as the matrix. The control standard endotoxin (CSE) extracted from Escherichia coli (E.coli O111:B4) was used as the target and the SALDI-MS method for the analysis of CSE was set up, which could be successfully applied to the rapid identification and quantitative analysis of endotoxin in different injections and drinking water. Compared with the traditional method of tachypleus amebocyte lysate (TAL), the MnO₂/G nanocomposites-based SALDI-MS method is of shows high sensitivity, high mass resolution, rapid analysis, high throughput, and good salt tolerance. The successful applications suggest the potential of the SALDI-MS for the rapid high-throughput screening of CSE in foods and drugs.