Extraction Method of Total Deoxyribo Nucleic Acid from Desert Plants

An extraction method of total deoxyribo nucleic acid(DNA) from desert plants has been established. The steps of the method are as follows:add a small amount of PVPP powder to the leaves of desert plants and grind them for more than three times to get the ultra-fine powder of the sample, immediately add the pre-treatment buffer solution to the sample powder, mix them evenly and centrifuge them at low speed, then discard the supernatant and leave the precipitate, add an equal volume of preheated extraction cracking solution to the precipitate, mix them evenly on a warm bath at 60~70℃ for one hour, extract the supernatant using high-speed centrifugation. And then add the purified mixture to the solution and mix, extract and centrifuge, extract the supernatant of the mixture again, add the precooled isopropanol and precipitate DNA at -18℃ for more than half an hour, after that, centrifuge the isopropanol solution in high-speed, wash the precipitate twice with 70% ethanol, air dry and to dissolve the precipitate with ultra-pure water. The advantages of the technology are simple operation and less time-consuming, and the operation process is about 2~3 h. The yield of DNA was higher than 500 ng/μL. This method has the advantages of high purity, good integrity and wide applicability.