Determination of Linoleic Acid in Grain by Underivatized High Performance Liguid Chromatography

An underivatized HPLC determination method for the Linoleic acid (LA) from grain was reported. The sample was analyzed in a C8 reversed phase column with the mobile phase acetonitrile+tetrahydrofuran+water (0.4% acetic acid) in ration (62:3:35) using refractive index detector. LA of a batch grain was determined. The content, standard derivation, variation coefficient were 3.16%, 0.13% and 4.13%, respectively. The reovrery reached 93.9%～96.22%.