Enantioseparation of Chiral Drug with High Performance Capillary Electrophoresis Using Kanamycin as Chiral Selector

A rapid enantioseparation method for racemic compounds by using kanamycin as a new chiral selector was set up. Two racemic drugs, BPME1 and BPME2, were successfully separated by capillary zone electrophoresis under the optimum separation conditions. The pH value of the background electrolyte, the concentration of chiral selector, the concentration both borax and phosphate were optimized. The effects of organic modifiers on chiral drug resolution were investigated. The use of borax and isopropyl alcohol was found to be effective for the chiral separation.